![Western Blot: MBP Antibody [NBP2-50035] - Analysis of different tissue lysates using chicken pAb to MBP, NBP2-50035, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] rat sciatic nerve, [5] mouse brain, and [6] mouse spinal cord. Multiple bands between 14-21.5kDa correspond to various alternate transcripts of the single MBP gene.](http://images.novusbio.com/fullsize/MBP-Antibody-NBP2-50035-img0002.jpg "Western Blot: MBP Antibody [NBP2-50035] - Analysis of different tissue lysates using chicken pAb to MBP, NBP2-50035, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] rat sciatic nerve, [5] mouse brain, and [6] mouse spinal cord. Multiple bands between 14-21.5kDa correspond to various alternate transcripts of the single MBP gene.")
| Reactivity | Hu, Mu, Rt, Po, BvSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Chicken |
| Conjugate | Unconjugated |
| Immunogen | Purified myelin basic protein isolated from bovine brain. [UniProt# P02687] |
| Isotype | IgY |
| Clonality | Polyclonal |
| Host | Chicken |
| Gene | MBP |
| Purity | IgY purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | In Western blot, this antibody recognizes all four CNS isotypes of rat MBP with bands at 21.5 kDa, 18.5kDa, 17kDa and 14kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C. |
| Buffer | PBS. Supplied as concentrated total IgY preparation from egg yolk. Exact concentration of target specific IgY is not quantifiable as the preparation contains both immune IgY specific for the target and also irrelevant, non-immune IgY. |
| Preservative | 0.035% Sodium Azide |
| Purity | IgY purified |
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| Publication using NBP2-50035 | Applications | Species |
|---|---|---|
| Sun Y, Winters A, Wang L et al. Metabolic Heterogeneity of Cerebral Cortical and Cerebellar Astrocytes Life (Basel, Switzerland) 2023-01-08 [PMID: 36676133] (WB, Mouse) Details: Dilution used in WB 1:5,000 |
WB | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for MBP Antibody (NBP2-50035)Find related products by research area.
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| Gene Symbol | MBP |
![Immunohistochemistry: MBP Antibody [NBP2-50035] - Analysis of rat brain cerebellum section stained with chicken pAb to Myelin Basic Protein (MBP), NBP2-50035, dilution 1:5,000 in green, and costained with rabbit pAb to NF-L, NBP1-05218, dilution 1:5,000 in red. The MBP antibody stains oligodendrocytes and myelin sheathes around axons. NBP1-05218 antibody labels dendrites and axons of neuronal cells.](http://images.novusbio.com/fullsize/MBP-Antibody-NBP2-50035-img0001.jpg "Immunohistochemistry: MBP Antibody [NBP2-50035] - Analysis of rat brain cerebellum section stained with chicken pAb to Myelin Basic Protein (MBP), NBP2-50035, dilution 1:5,000 in green, and costained with rabbit pAb to NF-L, NBP1-05218, dilution 1:5,000 in red. The MBP antibody stains oligodendrocytes and myelin sheathes around axons. NBP1-05218 antibody labels dendrites and axons of neuronal cells.")
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