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MBP Antibody (CL2827)

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Western Blot: MBP Antibody (CL2827) [NBP2-46631] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue
Western Blot: MBP Antibody (CL2827) [NBP2-46631] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue
Immunocytochemistry/ Immunofluorescence: MBP Antibody (CL2827) [NBP2-46631] - Staining of A549 cells using the Anti-MBP monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and ...read more
Immunohistochemistry: MBP Antibody (CL2827) [NBP2-46631] - Staining of mouse striatum shows strong positivity in myelinated fibers.
Western Blot: MBP Antibody (CL2827) [NBP2-46631] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue
Immunohistochemistry: MBP Antibody (CL2827) [NBP2-46631] - Staining of rat striatum shows strong immunoreactivity in myelinated neural fibers.
Immunohistochemistry: MBP Antibody (CL2827) [NBP2-46631] - Staining of mouse cerebral cortex shows strong immunoreactivity in myelinated fibers.
Immunohistochemistry: MBP Antibody (CL2827) [NBP2-46631] - Staining of human cerebral cortex shows strong immunoreactivity in myelinated fibers.
Immunohistochemistry: MBP Antibody (CL2827) [NBP2-46631] - Staining of rat cerebral cortex shows strong immunoreactivity in myelinated fibers.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
CL2827
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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MBP Antibody (CL2827) Summary

Immunogen
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence DENPVVHFFKNIVTPRTPPPSQGKGRGLSLSRFSWGAEGQRPGFGYGGRASDYKSAHKGFKGVDAQGTLSKIFKLGGRDSRSGSPM
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MBP
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for MBP Antibody (CL2827)

  • Hmbpr
  • MBP
  • MGC99675
  • mld
  • Myelin A1 protein
  • myelin basic protein
  • Myelin membrane encephalitogenic protein
  • shi

Background

Myelin Basic Protein (MBP) functions in the nervous system in myelination and is expressed in oligodendrocytes following differentiation. There are numerous isoforms generated by differential splicing events and post-translational modifications that have specialized functions. These functions include participation in signaling pathways prior to myelination, myelination or the re-myelination process following neural injury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MBP
Entrez
Uniprot