Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-87855] - Analysis in human cell line HEK 293.
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-87855] - Staining of human cell line U-2 OS shows positivity in nucleoli.
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-87855] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-87855] - Staining of human cerebellum shows strong positivity in nucleoli in Purkinje cells.
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-87855] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-87855] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
This antibody was developed against Recombinant Protein corresponding to amino acids: DVFVRKFQPERYKLWKAGKDNTVIDHTLPTPEAAEFLKESELPPRAGNEEECPEEDMEGVEDGEEGDLKTSLAKHRIGTKRHRVCLEIPQEVSQSELFPKEDLSSEQYEMTEC
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KDM4A
Purity
Immunogen affinity purified
Innovator's Reward
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WB reported in scientific literature (PMID: 25336657). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%). Mouse reactivity reported in the scientific literature (PMID: 25336657).
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified
Alternate Names for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody
EC 1.14.11
EC 1.14.11.-
JHDM3A
JHDM3AJMJD2
JmjC domain-containing histone demethylation protein 3A
JMJD2
JMJD2A
JMJD2Alysine-specific demethylase 4A
jumonji domain containing 2
jumonji domain containing 2A
Jumonji domain-containing protein 2A
KDM4A
KIAA0677jumonji C domain-containing histone demethylase 3A
Lysine (K)specific Demethylase 4A
Lysine (K)-specific Demethylase 4A
Background
JMJD2A is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein with a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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