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LMAN1 Antibody - Azide and BSA Free

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Western Blot: LMAN1 Antibody [NBP3-04910] - Analysis of extracts of various cell lines, using LMAN1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: ...read more
Immunocytochemistry/ Immunofluorescence: LMAN1 Antibody [NBP3-04910] - Analysis of U-2 OS cells using LMAN1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. LMAN1 antibody
Immunocytochemistry/ Immunofluorescence: LMAN1 Antibody [NBP3-04910] - Analysis of C6 cells using LMAN1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. LMAN1 antibody
Immunocytochemistry/ Immunofluorescence: LMAN1 Antibody [NBP3-04910] - Analysis of NIH-3T3 cells using LMAN1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. LMAN1 antibody

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free

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LMAN1 Antibody - Azide and BSA Free Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 270-480 of human LMAN1 (NP_005561.1). PGKEPPTPDKEISEKEKEKYQEEFEHFQQELDKKKEEFQKGHPDLQGQPAEEIFESVGDRELRQVFEGQNRIHLEIKQLNRQLDMILDEQRRYVSSLTEEISKRGAGMPGQHGQITQQELDTVVKTQHEILRQVNEMKNSMSETVRLVSGMQHPGSAGGVYETTQHFIDIKEHLHIVKRDIDNLVQRNMPSNEKPKCPELPPFPSCLSTVH
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LMAN1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.01% Thimerosal
Purity
Affinity purified

Alternate Names for LMAN1 Antibody - Azide and BSA Free

  • ERGIC-53
  • ERGIC53ER-Golgi intermediate compartment 53 kDa protein
  • F5F8Dendoplasmic reticulum-golgi intermediate compartment protein 53
  • FMFD1
  • Gp58
  • intracellular mannose specific lectin
  • Intracellular mannose-specific lectin MR60
  • Lectin mannose-binding 1
  • lectin, mannose-binding, 1
  • MCFD1coagulation factor V-factor VIII combined deficiency
  • MR60
  • protein ERGIC-53

Background

Protein trafficking along the endoplasmic reticulum (ER) to the Golgi apparatus occurs by vesicular transport between membranous compartments. LMAN1 is a mannose-specific lectin transport receptor protein which cycles between the ER and the Golgi complex. LMAN1 is involved in intracellular transport of many glycoproteins. LMAN1 is useful in studying mechanisms underlying protein trafficking early in the secretory pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LMAN1