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Lightning-Link® AMCA Antibody Labeling Kit

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Product Details

Summary
Conjugate
AMCA
Format
AMCA (A=350, E=450)
Kit Type
Antibody Labeling Kit

Order Details

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Lightning-Link® AMCA Antibody Labeling Kit Summary

Description
Lightning-Link Rapid is an innovative technology that enables direct labeling of proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits (See protocol for further information).

The easy-to-use, one step procedure allows researchers to covalently label biomolecules with only 30 seconds hands-on time; furthermore conjugates are ready to use in less than twenty minutes.

The researcher simply pipettes the biomolecule into a vial of lyophilized mixture containing the label of interest and incubates (for more details please watch the video below).

FeaturesBenefits
Quick and easy to useSave time, no special knowledge required
No separation steps100% recovery - no antibody/protein loss
Can be used in a wide range of applicationsFlexible
Freeze driedShips at ambient temperature, long shelf-life
Fully scalable (10 ug to 1 g or more)Easy transfer from R&D to manufacturing
Stringently QC testedConsistent high quality, excellent batch-to-batch reproducibility
Large number of labels available Experimental flexibility
Reliable: nearly 300 referencesSuccessfully used in many fields of research


Unlike standard AMCA labeling procedures, where a large molar excess of AMCA is employed, the Lightning-Link AMCA kit combines a low molar ratio of AMCA:antibody and high labeling efficiency to eliminate the need for purification steps.

AMCA (aminomethylcoumarin acetate) is a blue fluorescent dye commonly used in fluorescence microscopy, arrays and in-situ hybridisation. It has an excitation wavelength of 352nm and a maximal emission of 452nm.

Learn more about Lightning-Link™ Conjugation Kits by reading FAQs

For more information please check out these useful links!
Antibody Labeling Guide
Antibody Conjugation Illustrated Assay

Kit Type
Antibody Labeling Kit

Applications/Dilutions

Application Notes
By circumventing the desalting or dialysis steps that commonly interrupt traditional antibody conjugation procedures, LightningLink technology can be used to label both small (e.g. 10 ug) and large quantities of primary antibodies with ease. Batch-to-batch variation upon scale up is minimal as the process is so simple, and recoveries are always 100%.

Packaging, Storage & Formulations

Storage
Store at -20C.

Kit Components

Components
  1. 1 or 3 glass vial(s) of Lightning-Link® Rapid mix
  2. 1 vial of LL Rapid Modifier reagent
  3. 1 vial of LL Rapid Quencher reagent

Notes

This product is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

Background

Easy AMCA labeling of your antibody with 30 seconds hands-on time. The Lightning-Link conjugation system represents a quantum leap forward in conjugation technology. It allows you to make antibody conjugates with minimal hands-on time - less than 30 seconds. Lightning-Link simplifies immunoassay techniques, such as western blotting, ELISA and immunohistochemistry, by eliminating secondary reagents and cutting the number of incubation and wash steps. Despite its simplicity, Lightning-Link is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled fashion, creating high quality conjugates. As the procedure is not interrupted by desalting steps, trial conjugates can be prepared with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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