Lightning-Link® AMCA Antibody Labeling Kit Summary
Description |
Lightning-Link Rapid is an innovative technology that enables direct labeling of proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits (See protocol for further information). The easy-to-use, one step procedure allows researchers to covalently label biomolecules with only 30 seconds hands-on time; furthermore conjugates are ready to use in less than twenty minutes. The researcher simply pipettes the biomolecule into a vial of lyophilized mixture containing the label of interest and incubates (for more details please watch the video below). Features | Benefits | Quick and easy to use | Save time, no special knowledge required | No separation steps | 100% recovery - no antibody/protein loss | Can be used in a wide range of applications | Flexible | Freeze dried | Ships at ambient temperature, long shelf-life | Fully scalable (10 ug to 1 g or more) | Easy transfer from R&D to manufacturing | Stringently QC tested | Consistent high quality, excellent batch-to-batch reproducibility | Large number of labels available | Experimental flexibility | Reliable: nearly 300 references | Successfully used in many fields of research |
Unlike standard AMCA labeling procedures, where a large molar excess of AMCA is employed, the Lightning-Link AMCA kit combines a low molar ratio of AMCA:antibody and high labeling efficiency to eliminate the need for purification steps. AMCA (aminomethylcoumarin acetate) is a blue fluorescent dye commonly used in fluorescence microscopy, arrays and in-situ hybridisation. It has an excitation wavelength of 352nm and a maximal emission of 452nm. Learn more about Lightning-Link™ Conjugation Kits by reading FAQsFor more information please check out these useful links! Antibody Labeling Guide Antibody Conjugation Illustrated Assay |
Kit Type |
Antibody Labeling Kit |
Applications/Dilutions
Application Notes |
By circumventing the desalting or dialysis steps that commonly interrupt traditional antibody conjugation procedures, LightningLink technology can be used to label both small (e.g. 10 ug) and large quantities of primary antibodies with ease. Batch-to-batch variation upon scale up is minimal as the process is so simple, and recoveries are always 100%. |
Packaging, Storage & Formulations
Kit Components
Components
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- 1 or 3 glass vial(s) of Lightning-Link® Rapid mix
- 1 vial of LL Rapid Modifier reagent
- 1 vial of LL Rapid Quencher reagent
|
Notes
This product is manufactured by Abcam and distributed by Novus Biologicals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.
Background
Easy AMCA labeling of your antibody with 30 seconds hands-on time. The Lightning-Link conjugation system represents a quantum leap forward in conjugation technology. It allows you to make antibody conjugates with minimal hands-on time - less than 30 seconds. Lightning-Link simplifies immunoassay techniques, such as western blotting, ELISA and immunohistochemistry, by eliminating secondary reagents and cutting the number of incubation and wash steps. Despite its simplicity, Lightning-Link is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled fashion, creating high quality conjugates. As the procedure is not interrupted by desalting steps, trial conjugates can be prepared with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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