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Lgr5/GPR49 Antibody (707042)

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NS0 mouse myeloma cell line transfected with human Lgr5/GPR49 and eGFP was stained with and either (A) Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
NS0 mouse myeloma cell line transfected with human Lgr5/GPR49 and eGFP was stained with and either (A) Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078) or (B) Mouse IgG2A Isotype Control (Catalog # ...read more
Lgr5/GPR49 was detected in immersion fixed NSO mouse myeloma cell line transfected with GFP (green) tagged human LGR5 using Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078) at 10 µg/mL for 3 hours at ...read more
Lgr5/GPR49 was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with GFP (green) tagged mouse LGR5 using Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078) at 10 µg/mL ...read more
Lgr5/GPR49 was detected in MYC tagged human transfectants using Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078). Cells were stained using an anti-mouse HRP-conjugated secondary antibody (brown). ...read more
Lgr5/GPR49 was detected in immersion fixed MYC tagged mouse transfectants using Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078). Cells were stained using an anti-mouse HRP-conjugated secondary ...read more
Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078) induces TOPflash activity in the HEK293 human embryonic kidney cell line stably expressing LGR5 in the presence of Wnt-3a, but in the absence of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready, Func, ICC/IF
Clone
707042
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB8078U-100UG
Alexa Fluor 405
FAB8078V-100UG
Alexa Fluor 488
FAB8078G-100UG
Alexa Fluor 532
FAB8078X
Alexa Fluor 594
FAB8078T-100UG
Alexa Fluor 647
FAB8078R-100UG
Alexa Fluor 700
FAB8078N-100UG
Alexa Fluor 750
FAB8078S-100UG
Allophycocyanin
FAB8078A
Allophycocyanin/Cy7
FAB8078APCCY7
Biotin
FAB8078B
CoraFluor 1
FAB8078CL1
DyLight 350
FAB8078D
DyLight 405
FAB8078E
DyLight 488
FAB8078K
DyLight 550
FAB8078L
DyLight 594
FAB8078M
DyLight 650
FAB8078W
DyLight 680
FAB8078Y
DyLight 755
FAB8078Z
FITC
FAB8078F
HRP
FAB8078H
Janelia Fluor 525
FAB8078JF525
Janelia Fluor 549
FAB8078I
Janelia Fluor 646
FAB8078J
Janelia Fluor 669
FAB8078JF669
PE/Atto594
FAB8078PEATT594
PE/Cy5.5
FAB8078PECY55
PE/Cy7
FAB8078PECY7
PerCP
FAB8078C
Phycoerythrin
FAB8078P
mFluor Violet 450 SE
FAB8078MFV450
mFluor Violet 500 SE
FAB8078MFV500
mFluor Violet 610 SE
FAB8078MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB8078P
Carrier Free
FAB8078A
Carrier Free
MAB8078-SP
Carrier Free
FAB8078V-100UG
Carrier Free
FAB8078U-100UG
Carrier Free
FAB8078T-100UG
Carrier Free
FAB8078S-100UG
Carrier Free
FAB8078R-100UG
Carrier Free
FAB8078N-100UG
Carrier Free
FAB8078G-100UG
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Lgr5/GPR49 Antibody (707042) Summary

Additional Information
Published as Clone RD42
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Lgr5/GPR49
Met1-Ile560
Accession # O75473
Specificity
Detects human Lgr5/GPR49 in ELISA. Stains human and mouse Lgr5 transfected cells but not irrelevant transfectants in Flow Cytometry and Immunocytochemistry. This antibody, also known as "RD42", has been found to detect an epitope in C-terminal LRR cap of LGR5 (Ref. 1).
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
LGR5
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Agonist Activity
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publications using
MAB8078 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lgr5/GPR49 Antibody (707042)

  • FEX
  • G protein-coupled receptor 49
  • GPR49
  • GPR49G-protein coupled receptor HG38
  • GPR67
  • G-protein coupled receptor 49
  • G-protein coupled receptor 67
  • GRP49
  • HG38
  • leucine-rich repeat containing G protein-coupled receptor 5
  • leucine-rich repeat-containing G protein-coupled receptor 5
  • leucine-rich repeat-containing G-protein coupled receptor 5
  • Lgr5
  • MGC117008
  • orphan G protein-coupled receptor HG38

Background

Leucine‑rich repeat G‑protein‑coupled receptor 5 (Lgr5), also called GPR49, is a 907 amino acid (aa), approximately 97 kDa (calculated), seven‑transmembrane glycoprotein receptor in the Lgr family of cell surface receptors. The subfamily of Lgrs comprising Lgr4, Lgr5, and Lgr6 are G‑protein‑independent mediators of the potentiating effect of R‑Spondins on Wnt signaling (2). Lgr5 binds and forms complexes with R‑Spondins, Frizzled Wnt receptors and LRP Wnt co‑receptors. The region of the human Lgr5 long extracellular domain used as an immunogen shares 90% amino acid sequence identity with mouse and rat Lgr5, respectively. Lgr5 is found on embryonic and adult epithelial stem cells (3). Lgr5+ stem cells can produce all epithelial cell types of the intestinal crypts (4). Abnormal LGR5 expression and regulation in stem cells might give rise to cancers such as intestinal, hepatocellular, pancreatic and ovarian carcinomas (5,6). This antibody has been referred to as "RD42" in Peng et al. (1).
  1. Peng et al. (2013) Cell Rep. 3(6):1885.
  2. de Lau et al. (2014) Genes Dev. 28(4):305.
  3. Barker et al. (2013) Development 140(12):2484.
  4. Clevers (2013) Nature 495(7439):53.
  5. Wu et al. (2014) Nat Commun 5:3149.
  6. Jang et al. (2013) PLoS One 8(12): e82390495.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Lgr5/GPR49
Antibody (707042)
MAB8078
Lgr5/GPR49 Antibody (707042)
Species: Hu
Applications: Flow, CyTOF-ready, Func, ICC/IF

Publications for Lgr5/GPR49 Antibody (MAB8078)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: IHC, IHC-P, Immunohistochemistry, Western Blot.


Filter By Application
IHC
(1)
IHC-P
(1)
Immunohistochemistry
(1)
Western Blot
(1)
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(4)
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Bioinformatics

Gene Symbol LGR5
Uniprot