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Kell Antibody (214711)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clone
214711
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB1454-SP
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Kell Antibody (214711) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Kell
Phe50-Trp713
Accession # Q9EQF2
Specificity
Detects mouse Kell in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) ECE-2, rhNeprilysin, or recombinant mouse Neprilysin is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
KEL
Purity Statement
Protein A or G purified from hybridoma culture supernatant
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Kell Antibody (214711)

  • CD238 antigen
  • CD238
  • EC 3.4.24.-
  • ECE3
  • KEL
  • kell blood group antigen
  • kell blood group glycoprotein
  • Kell blood group
  • Kell blood group, metalloendopeptidase
  • Kell blood group, metallo-endopeptidase
  • Kell

Background

Kell, a type II membrane glycoprotein, is linked through a single disulfide bond to XK, a putative membrane transporter. The two proteins constitute the Kell blood group antigens (1). Kell is a zinc endopeptidase of the neprilysin (NEP) family which also includes endothelin converting enzymes (ECE-1 and ECE-2), PEX, XCE, DINE and several NEP-like proteins (2). It has been shown to cleave big endothelin-3 (ET-3) at Trp21-Ile22, yielding ET-3, and to a much lesser extent, big ET-1 and big ET-2 at Trp21-Val22, yielding ET-1 and ET-2 (3). Several different molecular defects cause the Kell null phenotype, which has no obvious clinical outcome (4).

  1. Lee, S. et al. (2000) Transfus. Med. Rev. 14:93.
  2. Turner, A.J. et al. (2001) BioEssays. 23:261.
  3. Lee, S. et al. (1999) Blood 94:1440.
  4. Lee, S. et al. (2001) J. Biol. Chem. 276:27281.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Kell Antibody
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MAB1454
Kell Antibody (214711)
Species: Mu
Applications: WB

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Bioinformatics

Gene Symbol KEL
Uniprot