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Integrin alpha V/CD51 Antibody

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Western Blot: Integrin alpha V/CD51 Antibody [NBP2-16973] - A549 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin alpha V antibody [C2C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Western Blot: Integrin alpha V/CD51 Antibody [NBP2-16973] - A549 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin alpha V antibody [C2C3], C-term ...read more
Immunohistochemistry-Paraffin: Integrin alpha V/CD51 Antibody [NBP2-16973] - Human colon carcinoma, using Integrin alpha V antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, ...read more
Western Blot: Integrin alpha V/CD51 Antibody [NBP2-16973] - Sample (30 ug of whole cell lysate) A: HUVEC 7. 5% SDS PAGE gel, diluted at 1:500.
Western Blot: Integrin alpha V/CD51 Antibody [NBP2-16973] - Sample (30 ug of whole cell lysate) A: RD (Rhabdomyosarcoma) cell (Mock control) B: RD transfected scramble siRNA C: RD transfected ITGAV siRNA 8% SDS PAGE ...read more

Product Details

Summary
Reactivity Hu, Mu, Po, Bv, RM, ShSpecies Glossary
Applications WB, ELISA, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Integrin alpha V/CD51 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Integrin alpha V/CD51. The exact sequence is proprietary.
Localization
Membrane; Single-pass type I membrane protein
Predicted Species
Porcine (92%), Bovine (91%), Rhesus Macaque (99%), Sheep (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ITGAV
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA Assay dependent
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockdown Validated
  • Western Blot 1:500-1:3000
Theoretical MW
116 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-16973.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris-Glycine (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for Integrin alpha V/CD51 Antibody

  • antigen identified by monoclonal L230
  • CD51 antigen
  • CD51
  • Integrin alpha V
  • integrin alpha-V
  • integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
  • ITGAV
  • MSK8
  • Vitronectin Receptor alpha
  • Vitronectin receptor subunit alpha
  • VNRA
  • VNRADKFZp686A08142

Background

ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Bioinformatics

Gene Symbol ITGAV