Integrin alpha V/CD51 Antibody (P2W7) - Azide and BSA Free Summary
Immunogen |
An ocular melanoma cell line (V+B2) expressing high levels of human integrin alpha V beta 2. |
Localization |
Type I membrane protein. |
Isotype |
IgG1 |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
ITGAV |
Purity |
Protein A purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- CyTOF-ready
- Flow Cytometry 0.25 ug/10^6 cells
- Immunocytochemistry/ Immunofluorescence 8-25 ug/ml
- Immunohistochemistry 1:10-1:500
- Immunoprecipitation 1:10-1:500
- Western Blot 1:100-1:2000
|
Application Notes |
Human alpha v is about 150-160kDa under non-reducing conditions. Under reducing conditions it is cleaved into 2 bands of about 125-130kDa +20-25kDa. This antibody is CyTOF ready. |
Theoretical MW |
155 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
Not yet tested in other species.
Packaging, Storage & Formulations
Storage |
Store at 4C. Do not freeze. |
Buffer |
PBS |
Preservative |
No Preservative |
Concentration |
1 mg/ml |
Purity |
Protein A purified |
Alternate Names for Integrin alpha V/CD51 Antibody (P2W7) - Azide and BSA Free
Background
Integrin alpha V chain interacts with the integrin beta 3 subunit/CD61 to form the alpha-V-beta-3 heterodimer/vitronectin receptor. It is expressed on endothelial cells, some activated leukocytes, NK cells, macrophages, neutrophils, and platelets. Integrin alpha V also forms heterodimers with the integrin beta 1, beta 5, beta 6, and beta 8 subunits. Alpha-V-beta-3 is an activation dependent receptor for platelet attachment and spreading on vitronectin and other matrix components.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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