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ImmPRESS™ Excel Amplified anti-Rabbit IgG HRP Polymer Staining Kit (Colorimetric)

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Immunohistochemistry: ImmPRESS(TM) Excel anti-Rabbit IgG Staining Kit (Colorimetric) [MP-7601-NB]

Product Details

Summary
Reactivity RbSpecies Glossary
Applications ELISA, Func, ICC/IF, IHC
Conjugate
HRP Polymer

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Catalog# & Conjugate Size Price
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ImmPRESS™ Excel Amplified anti-Rabbit IgG HRP Polymer Staining Kit (Colorimetric) Summary

Description
Features:
  • Complete staining kits
  • Highest sensitivity
  • Precise, sharp antigen localization
  • Low background
  • Amplified polymer technology
  • Convenient prediluted, ready-to-use reagents
  • Specificity
    ImmPRESS(TM) Excel Staining Kit (15 ml) will stain approximately 75-150 sections and an ImmPRESS(TM) Excel Staining Kit (50 ml) will stain approximately 250-500 sections.
    Kit Type
    Staining Kit (Colorimetric)

    Applications/Dilutions

    Dilutions
    • ELISA
    • Immunocytochemistry/ Immunofluorescence
    • Immunohistochemistry
    • In-situ Hybridization
    Application Notes
    .

    Packaging, Storage & Formulations

    Storage
    Store at 4C. Do not freeze.

    Kit Components

    Components
    1. 2.5% Normal serum for blocking non-specific binding (r.t.u.)
    2. Amplifier(TM) Antibody (either goat anti-mouse IgG or goat anti-rabbit IgG)
    3. BLOXALL(TM) Endogenous HRP/AP Blocking Solution (r.t.u.)
    4. ImmPACT(TM) DAB EqV Substrate
    5. ImmPRESS(TM) Excel Amplified HRP Polymer Reagent (anti-goat IgG)

    Notes

    This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

    Background

    The ImmPRESS(TM) Excel Amplified HRP (peroxidase) Polymer Staining Kit is an enzymatic, amplification system that produces crisp, highly sensitive, specific staining with low background. The ImmPRESS(TM) Excel Amplified Staining System capitalizes on all the advantages of the ImmPRESS(TM) polymerized reporter enzyme staining system technology but offers additional sensitivity and convenience.
    This system employs a proprietary ready-to-use (RTU) Amplifier Antibody, followed by a ready-to-use (RTU) ImmPRESS(TM) Excel HRP Polymer Reagent. These reagents are affinity purified and extensively cross-adsorbed to ensure high sensitivity and low background. In addition, the ImmPRESS(TM) Excel Reagent is conjugated to horseradish peroxidase micropolymers, a novel approach that avoids the use of large dextrans or other macromolecules as a backbone. These unique micropolymers allow a higher density of enzymes per antibody to bind the target with minimal interference. The result is an increase in target accessibility, signal intensity, and significantly less background staining.
    The included ImmPACT(TM) DAB EqV Substrate produces a crisp, dark brown reaction product with the excellent sensitivity and low background characteristic of the ImmPRESS(TM) /ImmPACT(TM) combination.

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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    Product General Protocols

    Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

    Video Protocols

    ICC/IF Video Protocol

    FAQs for ImmPRESS(TM) Excel Amplified anti-Rabbit IgG HRP Polymer Kit (MP-7601-NB) (0)

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