Home » ICAM-3/CD50 » ICAM-3/CD50 Antibodies » ICAM-3/CD50 Antibody (Cal 3.10) [Unconjugated]
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ICAM-3/CD50 Antibody (Cal 3.10) [Unconjugated]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready
Clone
Cal 3.10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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ICAM-3/CD50 Antibody (Cal 3.10) [Unconjugated] Summary

Immunogen
Recombinant human ICAM-3/CD50 Fc Chimera
Specificity
Detects human ICAM-3/CD50 in Western blots. Does not cross-react with recombinant human (rh) ICAM-1 or rhVCAM-1.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ICAM3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
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Applications/Dilutions

Dilutions
  • Adhesion Blockade
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL
Publications
Read Publication using
BBA29 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ICAM-3/CD50 Antibody (Cal 3.10) [Unconjugated]

  • CD50 antigen
  • CD50
  • CDW50CDw50
  • ICAM3
  • ICAM-3
  • ICAM-Rintercellular adhesion molecule-3
  • intercellular adhesion molecule 3

Background

Intercellular Adhesion Molecule-3 (ICAM-3), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and alpha d/ beta 2. ICAM-3 is expressed on leukocytes and epidermal Langerhans cells. It may play an important role in T cell stimulation by Langerhans cells. ICAM-3 has not been identified in mice.

  1. Hayflick, J. et al. (1998) Immunologic Res. 17:313.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ICAM-3/CD50 Antibody (BBA29)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Adhesion Assay, Direct ELISA.


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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ICAM3