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ICAM-1/CD54 Antibody (166623)

 (6 Publications)
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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA(Cap)
Clone
166623
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

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Catalog# & Conjugate Size Price
Phycoerythrin
FAB796P
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB796P
Carrier Free
MAB796-SP
Carrier Free
MAB796-500
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ICAM-1/CD54 Antibody (166623) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ICAM-1
Gln28-Asn485
Accession # P13597
Specificity
Detects mouse ICAM-1 in ELISAs and Western blots. In Western blots, this antibody shows weak cross-reactivity with recombinant human (rh) ICAM‑1 and rmICAM-2 and no cross-reactivity with rhCD31, rmDCC, rhICAM-2, rhICAM-3, rrICAM-1, rmMAdCAM-1, or rmVCAM-1.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
ICAM1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 1 ug/mL
Application Notes
ELISA Detection: Mouse ICAM-1/CD54 Biotinylated Antibody (Catalog number BAF796)
Standard: Recombinant Mouse ICAM-1/CD54 Fc Chimera (Catalog number 796-IC)
Publications
Read Publications using
MAB796 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ICAM-1/CD54 Antibody (166623)

  • BB2
  • CD54 antigen
  • CD54
  • cell surface glycoprotein P3.58
  • human rhinovirus receptor
  • ICAM1
  • ICAM-1
  • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
  • intercellular adhesion molecule 1
  • Major group rhinovirus receptor
  • P3.58

Background

Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma , TNF-alpha , IL-1 beta , and LPS. Mouse and human ICAM-1 share approximately 54% amino acid sequence identity. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer, and transplantation.

  1. Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book. Academic Press, p. 74.
  2. Siu, G. et al. (1989) J. Immunol. 143:3813.
  3. Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICAM-1/CD54
Antibody (166623)
MAB796
ICAM-1/CD54 Antibody (166623)
Species: Mu
Applications: WB, ELISA(Cap)

Publications for ICAM-1/CD54 Antibody (MAB796)(6)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: IHC, Luminex Development, Western Blot.


Filter By Application
IHC
(1)
Luminex Development
(1)
Western Blot
(3)
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Mouse
(5)
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Showing Publications 1 - 6 of 6.
Publications using MAB796 Applications Species
WC Liu, MC Yang, YY Wu, PH Chen, CM Hsu, LW Chen Lactobacillus plantarum reverse diabetes-induced Fmo3 and ICAM expression in mice through enteric dysbiosis-related c-Jun NH2-terminal kinase pathways PLoS ONE, 2018-05-31;13(5):e0196511. 2018-05-31 [PMID: 29851956] (Western Blot, Mouse) Western Blot Mouse
Stephanie Traub, Alexandra Nikonova, Alan Carruthers, Rebecca Dunmore, Katherine A. Vousden, Leila Gogsadze et al. An Anti-Human ICAM-1 Antibody Inhibits Rhinovirus-Induced Exacerbations of Lung Inflammation PLoS Pathogens 8/1/2013 [PMID: 23935498]
Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012-01-25;58(2):169-77. 2012-01-25 [PMID: 22277799] (Luminex Development, Mouse) Luminex Development Mouse
Mendez MP, Monroy YK, Du M Overexpression of sICAM-1 in the Alveolar Epithelial Space Results in an Exaggerated Inflammatory Response and Early Death in Gram Negative Pneumonia. Respir. Res., 2011-01-19;12(1):12. 2011-01-19 [PMID: 21247482] (Western Blot, Mouse) Western Blot Mouse
Haraguchi G, Suzuki J, Kosuge H, Ogawa M, Koga N, Muto S, Itai A, Kagechika H, Shudo K, Isobe M A new RXR agonist, HX630, suppresses intimal hyperplasia in a mouse blood flow cessation model. J. Mol. Cell. Cardiol., 2006-09-11;41(5):885-92. 2006-09-11 [PMID: 16963076] (IHC, Mouse) IHC Mouse
Mendez MP, Morris SB, Wilcoxen S, Greeson E, Moore B, Paine R Shedding of soluble ICAM-1 into the alveolar space in murine models of acute lung injury. Am. J. Physiol. Lung Cell Mol. Physiol., 2005-12-22;290(5):L962-70. 2005-12-22 [PMID: 16373671] (Western Blot, Mouse) Western Blot Mouse

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Bioinformatics

Gene Symbol ICAM1
Uniprot