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Rat Hepcidin Antimicrobial Peptide ELISA Kit (Colorimetric)

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ELISA: Rat Hepcidin Antimicrobial Peptide ELISA Kit (Colorimetric) [NBP2-82130] - Samples were spiked with high concentrations of Rat Hepcidin Antimicrobial Peptide and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat Hepcidin Antimicrobial Peptide ELISA Kit (Colorimetric) [NBP2-82130] - Samples were spiked with high concentrations of Rat Hepcidin Antimicrobial Peptide and diluted with Reference Standard & Sample Diluent ...read more
ELISA: Rat Hepcidin Antimicrobial Peptide ELISA Kit (Colorimetric) [NBP2-82130] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Cell supernatant
Standard Curve Range
78.13 - 5000 pg/mL
Sensitivity
46.88 pg/mL

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Rat Hepcidin Antimicrobial Peptide ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat Hepcidin Antimicrobial Peptide in samples. No significant cross-reactivity or interference between Rat Hepcidin Antimicrobial Peptide and analogues was observed.
Standard Curve Range
78.13 - 5000 pg/mL
Sensitivity
46.88 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 6%
Intra-Assay
CV% < 5%
Spike Recovery
average = 95% for serum; average = 97% for EDTA plasma; average = 97% for cell culture media
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
HAMP

Applications/Dilutions

Dilutions
  • ELISA
Publications
Read Publication using NBP2-82130.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Alternate Names for Rat Hepcidin Antimicrobial Peptide ELISA Kit (Colorimetric)

  • HAMP
  • HEPC
  • hepcidin antimicrobial peptide
  • Hepcidin
  • HEPCPutative liver tumor regressor
  • HFE2B
  • HFE2Bhepcidin
  • LEAP1
  • LEAP-1
  • LEAP1PLTR
  • Liver-expressed antimicrobial peptide 1
  • PLTR

Background

The product encoded by this gene is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity. Mutations in this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis, a disease caused by severe iron overload that results in cardiomyopathy, cirrhosis, and endocrine failure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for Hepcidin Antimicrobial Peptide ELISA Kit (NBP2-82130)(1)

We have publications tested in 1 application: ELISA.


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Product PDFs

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  Rat Hepcidin Antimicrobial Peptide ELISA Kit (Colorimetric) Manual
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Bioinformatics

Gene Symbol HAMP