Mouse Hepcidin Antimicrobial Peptide ELISA Kit (Colorimetric) Summary
Specificity |
This kit recognizes Mouse Hepcidin Antimicrobial Peptide in samples. No Significant cross-reactivity or interference between Mouse Hepcidin Antimicrobial Peptide and analogues was observed. |
Standard Curve Range |
125 - 8000 pg/mL |
Sensitivity |
75 pg/mL |
Assay Type |
Sandwich-ELISA |
Inter-Assay |
CV% < 6% |
Intra-Assay |
CV% < 5% |
Spike Recovery |
average = 94% for serum; average = 97% for EDTA plasma; average = 94% for cell culture media |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
HAMP |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab
|
Alternate Names for Mouse Hepcidin Antimicrobial Peptide ELISA Kit (Colorimetric)
Background
The product encoded by this gene is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity. Mutations in this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis, a disease caused by severe iron overload that results in cardiomyopathy, cirrhosis, and endocrine failure.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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