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GSG2 Antibody

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Biological Strategies: Western Blot: GSG2 Antibody [NBP1-26626] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified ...read more
Immunohistochemistry-Paraffin: GSG2 Antibody [NBP1-26626] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Haspin used at a dilution of 1:200 (1ug/ml). Detection: DAB

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
   

Biological Strategies

     

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GSG2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 300 and 350 of human serine/threonine-protein kinase Haspin (germ cell-specific gene 2) using the numbering given in entry NP_114171.2 (GeneID 83903).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HASPIN
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 5-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Theoretical MW
88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GSG2 Antibody

  • EC 2.7.11.1
  • germ cell associated 2 (haspin)
  • Germ cell-specific gene 2 protein
  • Haploid germ cell-specific nuclear protein kinase
  • haspin
  • H-haspin
  • serine/threonine-protein kinase haspin

Background

Hapsin is a serine-threonine protein kinase that was originally identified as a cDNA expressed specifically in mouse testicular germ cells. Haspin associates with the chromosomes, centrosomes, and spindle and is critical for proper chromosome alignment and segregation. Haspin is responsible for the phosphorylation of histone H3 at threonine 3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HASPIN
Entrez
Uniprot