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GRAP2 Antibody

Images

 
Western Blot: GRAP2 Antibody [NB100-805] - HEK293 overexpressing GRAP2 (RC206546) and probed with GRAP2 (mock transfection in first lane).
Western Blot: GRAP2 Antibody [NB100-805] - staining of Jurkat lysate (30ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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GRAP2 Antibody Summary

Immunogen
Peptide with sequence C-GLFPANYVAPMTR corresponding to C-Terminus according to NP_004801.1.
Predicted Species
Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GRAP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1:100-1:2000
Application Notes
WB: Approx. 38 kDa band observed Jurkat lysates (calculated MW of 37.9 kDa band according to NP_004801.1). In transfected HEK293 transiently expressing GRAP2 a band of approx. 40 kDa is observed. This band is not observed in the non-transfected HEK293.
Control
Jurkat Whole Cell Lysate
GRAP2 Overexpression Lysate
Publications
Read Publication using NB100-805.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GRAP2 Antibody

  • Adapter protein GRID
  • GADS
  • GADSSH3-SH2-SH3 adapter Mona
  • GRAP2
  • GRB2L
  • GRB-2-like protein
  • GRB2-related adapter protein 2
  • GRB2-related adaptor protein 2
  • GRB2-related protein with insert domain
  • GRBLG
  • GRBLGGRAP-2
  • GRBX
  • Grf40 adapter protein
  • Grf40
  • grf-40
  • GRID
  • GRIDGRPL
  • Growth factor receptor-binding protein
  • growth factor receptor-bound protein 2-related adaptor protein 2
  • GRPL
  • Hematopoietic cell-associated adapter protein GrpL
  • hematopoietic cell-associated adaptor protein GRPL
  • Mona
  • P38
  • Protein GADS
  • SH3-SH2-SH3 adaptor molecule

Background

This gene encodes a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains. Transcript variants utilizing alternative polyA sites exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA

Publications for GRAP2 Antibody (NB100-805)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for GRAP2 Antibody (NB100-805) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on GRAP2

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Bioinformatics

Gene Symbol GRAP2
Entrez
Uniprot