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GNAQ Antibody

Images

 
Western Blot: GNAQ Antibody [NBP1-31985] - A. 30 ug 293T whole cell extract B. 30 ug whole cell extract of 3xFlag-human GNAQ-transfected 293T cells 10 % SDS-PAGE GNAQ antibody dilution: 1:1000
Immunohistochemistry-Paraffin: GNAQ Antibody [NBP1-31985] - Paraffin-embedded mouse ovary. GNAQ antibody dilution: 1:500.
Western Blot: GNAQ Antibody [NBP1-31985] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GNAQ antibody diluted at 1:500.
Immunohistochemistry-Paraffin: GNAQ Antibody [NBP1-31985] - Paraffin-embedded placenta. GNAQ antibody dilution: 1:100.
Immunohistochemistry-Paraffin: GNAQ Antibody [NBP1-31985] - Paraffin-embedded rat intestine. GNAQ antibody diluted at 1:500.
Immunohistochemistry-Paraffin: GNAQ Antibody [NBP1-31985] - Paraffin-embedded mouse prostate. GNAQ antibody dilution: 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, RM, Xp, ZeSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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GNAQ Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human GNAQ. The exact sequence is proprietary.
Localization
N/A
Predicted Species
Porcine (100%), Bovine (100%), Zebrafish (93%), Rhesus Macaque (100%), Xenopus (96%), Chicken (98%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GNAQ
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (96%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for GNAQ Antibody

  • GAQG-ALPHA-q
  • guanine nucleotide binding protein (G protein), q polypeptide
  • Guanine nucleotide-binding protein alpha-q
  • guanine nucleotide-binding protein G(q) subunit alpha

Background

Guanine nucleotide-binding proteins are a family of heterotrimeric proteins that couple cell surface, 7-transmembrane domain receptors to intracellular signaling pathways. Receptor activation catalyzes the exchange of GTP for GDP bound to the inactive G protein alpha subunit resulting in a conformational change and dissociation of the complex. The G protein alpha and beta-gamma subunits are capable of regulating various cellular effectors. Activation is terminated by a GTPase intrinsic to the G-alpha subunit. G-alpha-q is the alpha subunit of one of the heterotrimeric GTP-binding proteins that mediates stimulation of phospholipase C-beta (MIM 600230).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GNAQ