![Western Blot: GM130/GOLGA2 Antibody [H00002801-B01P] - Analysis of GOLGA2 expression in human stomach.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Western-Blot-H00002801-B01P-img0007.jpg "Western Blot: GM130/GOLGA2 Antibody [H00002801-B01P] - Analysis of GOLGA2 expression in human stomach.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody reactive against mammalian transfected lysate. |
| Immunogen | GOLGA2 (AAH06381.1, 1 a.a. - 345 a.a.) full-length human protein. MQNDRTTISRALSQNRELKEQLAELQSGFVKLTNENMEITSALQSEQHVKRELGKKLGELQEKLSELKETVELKSQEAQSLQQQRDQYLGHLQQYVAAYQQLTSEKEVLHNQLLLQTQLVDQLQQQEAQGKAVAEMARQELQETQERLEAATQQNQQLRAQLSLMAHPGEGDGLDREEEEDEEEEEEAVAVPQPMPSIPEDLESREAMVAFFNSAVASAEEEQARLRGQLKEQSVRCRRLAHLLASAQKEPEAAAPAPGTGGDSVCGETHRALQGAMEKLQSRFMELMQEKADLKERPGRVLPVTTPLHSRSCSCFVRCRTPGSAQAWAATPAFLFFTGLTRMMR |
| Marker | Golgi Apparatus Marker |
| Specificity | GOLGA2 - golgi autoantigen, golgin subfamily a, 2, |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Mouse |
| Gene | GOLGA2 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. May also be used for IF, IHC-P. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4) |
| Preservative | No Preservative |
| Purity | Protein A purified |
![Simple Western Syntaxin 5 Antibody [Unconjugated]](https://images.novusbio.com/images2/Syntaxin_5_AF5687_Simple_Western_20944.jpg)
![Western Blot Syntaxin 5 Antibody [Unconjugated]](https://images.novusbio.com/images2/Syntaxin_5_AF5687_Western_Blot_5806.jpg)
![Immunohistochemistry Syntaxin 5 Antibody [Unconjugated]](https://images.novusbio.com/images2/Syntaxin_5_AF5687_Immunohistochemistry_19570.jpg)
| Publications using H00002801-B01P | Applications | Species |
|---|---|---|
| Fan SJ, Snell C, Turley H et al. PAT4 levels control amino-acid sensitivity of rapamycin-resistant mTORC1 from the Golgi and affect clinical outcome in colorectal cancer. Oncogene 2016-06-09 [PMID: 26434594] (WB) | WB | |
| Magnusson K, Appelqvist H, Cieslar-Pobuda A et al. Differential vital staining of normal fibroblasts and melanoma cells by an anionic conjugated polyelectrolyte Cytometry A. 2015-01-20 [PMID: 25605326] (ICC/IF, Human) | ICC/IF | Human |
| Wuensch T, Schulz S, Ullrich S et al. The peptide transporter PEPT1 is expressed in distal colon in rodents and Humans and contributes to water absorption. Am J Physiol Gastrointest Liver Physiol 2013-07-01 [PMID: 23660505] (ICC/IF, Human) | ICC/IF | Human |
| Goberdhan D, Fan SJ, Kroeger B, et al. Glutamine deprivation regulates the origin and function of cancer cell exosomes bioRxiv 2019-12-02 (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for GM130/GOLGA2 Antibody (H00002801-B01P)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [H00002801-B01P] - Rab1A (blue) is localised primarily on the cis-Golgi, labelled by the GM130 marker (red). Image collected and cropped by CiteAb from the following publication (nature.com/articles/onc2015363), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunocytochemistry-Immunofluorescence-H00002801-B01P-img0009.jpg "Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [H00002801-B01P] - Rab1A (blue) is localised primarily on the cis-Golgi, labelled by the GM130 marker (red). Image collected and cropped by CiteAb from the following publication (nature.com/articles/onc2015363), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [H00002801-B01P] - Analysis of purified antibody to GOLGA2 on formalin-fixed paraffin-embedded human esophagus. (antibody concentration 3 ug/ml)](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunohistochemistry-Paraffin-H00002801-B01P-img0006.jpg "Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [H00002801-B01P] - Analysis of purified antibody to GOLGA2 on formalin-fixed paraffin-embedded human esophagus. (antibody concentration 3 ug/ml)")
![Western Blot: GM130/GOLGA2 Antibody [H00002801-B01P] - Analysis of GOLGA2 expression in transfected 293T cell line by GOLGA2 polyclonal antibody. Lane 1: GOLGA2 transfected lysate(37.95 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Western-Blot-H00002801-B01P-img0008.jpg "Western Blot: GM130/GOLGA2 Antibody [H00002801-B01P] - Analysis of GOLGA2 expression in transfected 293T cell line by GOLGA2 polyclonal antibody. Lane 1: GOLGA2 transfected lysate(37.95 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [H00002801-B01P] - Analysis of purified antibody to GOLGA2 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/GM130-GOLGA2-Antibody-Immunocytochemistry-Immunofluorescence-H00002801-B01P-img0005.jpg "Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [H00002801-B01P] - Analysis of purified antibody to GOLGA2 on HeLa cell. (antibody concentration 10 ug/ml)")
![Flow Cytometry: Mouse IgG Isotype Control [NBP1-97019]](https://images.novusbio.com/images/Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP1-97019-img0004.jpg "Flow Cytometry: Mouse IgG Isotype Control [NBP1-97019]")