NBP1-49533 and NBP1-49533R are listed as being raised against a synthetic peptide made to an internal portion of the human GLUT4 protein (between residues 480-509 or 480-530 for NBP1-49533R), but since the protein is only 509aa in length, I am curious to know if the description as internal means that the actual sequence does not include the very c-terminal part, or if the listed sequence range is incorrect and the actual sequence in more internal?

Name: Glut4 Antibody [DyLight 550]
Brand: Novus Biologicals
SKU: NBP1-49533R
Price: 569 USD

Question

Accepted Answer

We highly appreciate your feedback on the immunogen info on the datasheet of our Glucose Transporter GLUT4 antibody #NBP1-49533, and yes, the immunogen sequence is from human Glucose Transporter GLUT4 protein between residues 480-509. Similarly, the sequence for Glucose Transporter GLUT4 Antibody [DyLight 550] (NBP1-49533R) would also be the same (between residues 480-509). It is certainly C-terminal region and we apologize for the mistake committed on our end about writing it as internal region.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, Rt, PlSpecies Glossary
Applications	WB, Flow, ICC/IF, IHC, KD
Clonality	Polyclonal
Host	Rabbit
Conjugate	DyLight 550

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NBP1-49533AF350

Alexa Fluor 405 NBP1-49533AF405

Alexa Fluor 488 NBP1-49533AF488

Alexa Fluor 532 NBP1-49533AF532

Alexa Fluor 594 NBP1-49533AF594

Alexa Fluor 647 NBP1-49533AF647

Alexa Fluor 700 NBP1-49533AF700

Alexa Fluor 750 NBP1-49533AF750

Allophycocyanin NBP1-49533APC

Allophycocyanin/Cy7 NBP1-49533APCCY7

Biotin NBP1-49533B

CoraFluor 1 NBP1-49533CL1

DyLight 350 NBP1-49533UV

DyLight 405 NBP1-49533V

DyLight 488 NBP1-49533G

DyLight 594 NBP1-49533DL594

DyLight 650 NBP1-49533C

DyLight 680 NBP1-49533FR

DyLight 755 NBP1-49533IR

FITC NBP1-49533F

HRP NBP1-49533H

Janelia Fluor 525 NBP1-49533JF525

Janelia Fluor 549 NBP1-49533JF549

Janelia Fluor 585 NBP1-49533JF585

Janelia Fluor 635 NBP1-49533JF635

Janelia Fluor 646 NBP1-49533JF646

Janelia Fluor 669 NBP1-49533JF669

PE NBP1-49533PE

PE/Atto594 NBP1-49533PEATT594

PE/Cy5.5 NBP1-49533PECY55

PE/Cy7 NBP1-49533PECY7

PerCP NBP1-49533PCP

Unconjugated NBP1-49533

mFluor Violet 450 SE NBP1-49533MFV450

mFluor Violet 500 SE NBP1-49533MFV500

mFluor Violet 610 SE NBP1-49533MFV610

Catalog# & Formulation	Size	Price
Standard Formulation NBP1-49533

BSA Free NBP1-49533SS

BSA Free NBP1-49533

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-49533AF350
Alexa Fluor 405	NBP1-49533AF405
Alexa Fluor 488	NBP1-49533AF488
Alexa Fluor 532	NBP1-49533AF532
Alexa Fluor 594	NBP1-49533AF594
Alexa Fluor 647	NBP1-49533AF647
Alexa Fluor 700	NBP1-49533AF700
Alexa Fluor 750	NBP1-49533AF750
Allophycocyanin	NBP1-49533APC
Allophycocyanin/Cy7	NBP1-49533APCCY7
Biotin	NBP1-49533B
CoraFluor 1	NBP1-49533CL1
DyLight 350	NBP1-49533UV
DyLight 405	NBP1-49533V
DyLight 488	NBP1-49533G
DyLight 594	NBP1-49533DL594
DyLight 650	NBP1-49533C
DyLight 680	NBP1-49533FR
DyLight 755	NBP1-49533IR
FITC	NBP1-49533F
HRP	NBP1-49533H
Janelia Fluor 525	NBP1-49533JF525
Janelia Fluor 549	NBP1-49533JF549
Janelia Fluor 585	NBP1-49533JF585
Janelia Fluor 635	NBP1-49533JF635
Janelia Fluor 646	NBP1-49533JF646
Janelia Fluor 669	NBP1-49533JF669
PE	NBP1-49533PE
PE/Atto594	NBP1-49533PEATT594
PE/Cy5.5	NBP1-49533PECY55
PE/Cy7	NBP1-49533PECY7
PerCP	NBP1-49533PCP
Unconjugated	NBP1-49533
mFluor Violet 450 SE	NBP1-49533MFV450
mFluor Violet 500 SE	NBP1-49533MFV500
mFluor Violet 610 SE	NBP1-49533MFV610

Glut4 Antibody [DyLight 550]

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Bioinformatics

Immunogen	A synthetic peptide from the human Glucose Transporter GLUT4 protein [UniProt P14672]
Localization	Endomembrane system; Multi-pass membrane protein. Cytoplasm - perinuclear region. Note: Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	SLC2A4
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Dilutions	Flow (Intracellular) Flow Cytometry Immunocytochemistry/ Immunofluorescence Immunohistochemistry Immunohistochemistry-Paraffin Knockdown Validated Western Blot
Application Notes	Optimal dilution of this antibody should be experimentally determined.

Storage	Store at 4C in the dark.
Buffer	50mM Sodium Borate
Preservative	0.05% Sodium Azide
Purity	Immunogen affinity purified