![Immunohistochemistry: Geminin Antibody [NB100-40826] - Detection of human Geminin by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Geminin (NB100-40826). Detection: DAB](http://images.novusbio.com/fullsize/Geminin-Antibody-Immunohistochemistry-NB100-40826-img0006.jpg "Immunohistochemistry: Geminin Antibody [NB100-40826] - Detection of human Geminin by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Geminin (NB100-40826). Detection: DAB")
| Reactivity | HuSpecies Glossary |
| Applications | IHC, IP, WB (-) |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 50 and 100 of human Geminin DNA replication Inhibitor using the numbering given in entry NP_056979.1 (GeneID 51053). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | GMNN |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NB100-40826 | Applications | Species |
|---|---|---|
| Yim H, Erikson RL. Polo-like kinase 1 depletion induces DNA damage in early S prior to caspase activation. Mol Cell Biol 2009-05-01 [PMID: 19289504] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Geminin Antibody (NB100-40826)Find related products by research area.
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| Gene Symbol | GMNN |
| Entrez |
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![Immunohistochemistry-Paraffin: Geminin Antibody [NB100-40826] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-Geminin used at a dilution of 1:250.](http://images.novusbio.com/fullsize/Geminin-Antibody-Immunohistochemistry-Paraffin-NB100-40826-img0001.jpg "Immunohistochemistry-Paraffin: Geminin Antibody [NB100-40826] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-Geminin used at a dilution of 1:250.")
![Immunohistochemistry: Geminin Antibody [NB100-40826] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Geminin used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Geminin-Antibody-Immunohistochemistry-NB100-40826-img0005.jpg "Immunohistochemistry: Geminin Antibody [NB100-40826] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Geminin used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB")
![Immunoprecipitation: Geminin Antibody [NB100-40826] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-Geminin antibody NB100-40826 used for IP at 3 mcg/mg lysate. Geminin was also immunoprecipitated by another rabbit anti-Geminin antibodies and NB100-40827, which recognize downstream epitopes.](http://images.novusbio.com/fullsize/Geminin-Antibody-Immunoprecipitation-NB100-40826-img0004.jpg "Immunoprecipitation: Geminin Antibody [NB100-40826] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-Geminin antibody NB100-40826 used for IP at 3 mcg/mg lysate. Geminin was also immunoprecipitated by another rabbit anti-Geminin antibodies and NB100-40827, which recognize downstream epitopes.")
![Immunoprecipitation: Geminin Antibody [NB100-40826] - Detection of human Geminin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Geminin antibody NB100-40826 used for IP at 3 ug per reaction. Geminin was also immunoprecipitated by rabbit anti-Geminin antibody NB100-40827. For blotting immunoprecipitated Geminin, NB100-40826 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Geminin-Antibody-Immunoprecipitation-NB100-40826-img0008.jpg "Immunoprecipitation: Geminin Antibody [NB100-40826] - Detection of human Geminin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Geminin antibody NB100-40826 used for IP at 3 ug per reaction. Geminin was also immunoprecipitated by rabbit anti-Geminin antibody NB100-40827. For blotting immunoprecipitated Geminin, NB100-40826 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")