![Immunocytochemistry/Immunofluorescence: GABA-AR alpha 1 Antibody (1F4) - Chimeric [NBP3-09047] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 1F4 (NBP3-09047) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09047, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/GABA-AR-alpha-1-Antibody-1F4-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09047-img0001.jpg "Immunocytochemistry/Immunofluorescence: GABA-AR alpha 1 Antibody (1F4) - Chimeric [NBP3-09047] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 1F4 (NBP3-09047) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09047, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
| Reactivity | HuSpecies Glossary |
| Applications | ICC/IF, WB |
| Clone | 1F4 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | This antibody was raised against alpha 1 beta 2 gamma 2 GABA-A receptor. |
| Isotype | IgG Kappa |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | GABRA1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques. FSEC: High affinity and specificity of the 1F4 antibody to alpha 1 beta 2 gamma 2 GABA-A receptor was demonstrated using fluorescence-detection size exclusion chromatography with GFP-tagged receptor (shift in elution volume) (Zhu et al., 2018). Crystallography: Furthermore, this antibody was utilised in production and purification of the alpha 1 beta 2 gamma 2 GABA-A receptor (Zhu et al., 2018). Finally, Fab fragments derived from the antibody were used to facilitate crystallisation and improve stabilisation of the alpha 1 beta 2 gamma 2 GABA-A receptor (Zhu et al., 2018). |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Proclin 300 |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for GABA-AR alpha 1 Antibody (NBP3-09047)Find related products by research area.
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| Gene Symbol | GABRA1 |
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