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Fibulin 1 Antibody (CL0337)

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Fibulin 1 Antibody (CL0337) [NBP2-52918] - Staining in human cervix, uterine and liver tissues. Corresponding FBLN1 RNA-seq data are presented for the same ...read more
Genetic Strategies: Western Blot: Fibulin 1 Antibody (CL0337) [NBP2-52918] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-FBLN1 antibody. Remaining relative ...read more
Western Blot: Fibulin 1 Antibody (CL0337) [NBP2-52918] - Lane 1: Marker [kDa] Lane 2: Human plasma
Immunohistochemistry-Paraffin: Fibulin 1 Antibody (CL0337) [NBP2-52918] - Staining of human uterine cervix shows strong positivity in the extracellular matrix.
Immunohistochemistry-Paraffin: Fibulin 1 Antibody (CL0337) [NBP2-52918] - Staining of human prostate shows moderate positivity in the extracellular matrix.
Immunohistochemistry-Paraffin: Fibulin 1 Antibody (CL0337) [NBP2-52918] - Staining of human breast cancer shows moderate positivity in the extracellular matrix of tumor stroma.
Immunohistochemistry-Paraffin: Fibulin 1 Antibody (CL0337) [NBP2-52918] - Staining of human liver shows no positivity as expected.
Staining of MCF7 cells using the Anti-FBLN1 monoclonal antibody, showing no specific staining of vesicles. MCF7 cells serves as a negative control based on RNA-seq values. Microtubule- and nuclear probes are visualized ...read more
Staining of RT-4 cells using the Anti-FBLN1 monoclonal antibody, showing specific staining in vesicles in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clone
CL0337
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Fibulin 1 Antibody (CL0337) Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: CESGIHNCLPDFICQNTLGSFRCRPKLQCKSGFIQDALGNCIDINECLSISAPCPIGHTCINTEGSYTCQKNVPNCGRGYHLNEEGTRCVDVDECAPPAEPCGKGHRCVNSPGSFRCECKTGYYFDGISRMCVDVNE
Predicted Species
Mouse (92%), Rat (93%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FBLN1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Knockdown Validated
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Fibulin 1 Recombinant Protein Antigen (NBP2-52918PEP)
Publications
Read Publication using
NBP2-52918 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for Fibulin 1 Antibody (CL0337)

  • FBLN
  • FBLN1
  • FIBL1
  • FIBL-1
  • Fibulin 1
  • fibulin-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Fibulin 1 Antibody (NBP2-52918)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IF/IHC.


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IF/IHC
(1)
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Mouse
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Fibulin 1 Antibody (NBP2-52918) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on Fibulin 1

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Bioinformatics

Gene Symbol FBLN1