![Western Blot: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A.](http://images.novusbio.com/fullsize/Fc-gamma-RIIA-CD32a-Antibody-9G5-Western-Blot-NBP1-47735-img0022.jpg "Western Blot: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clone | OTI9G5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.66 mg/ml |
| Immunogen | Full length human recombinant protein of human FCGR2A (NP_067674) produced in HEK293T cell. |
| Specificity | This antibody is specific for Homo sapiens Fc fragment of IgG, low affinity IIa, receptor (CD32) (FCGR2A), transcript variant 2. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | FCGR2A |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 34.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.66 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Fc gamma RIIA/CD32a Antibody (NBP1-47735)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FCGR2A.](http://images.novusbio.com/fullsize/Fc-gamma-RIIA-CD32a-Antibody-9G5-Immunocytochemistry-Immunofluorescence-NBP1-47735-img0013.jpg "Immunocytochemistry/Immunofluorescence: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FCGR2A.")
![Immunohistochemistry-Paraffin: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Staining of paraffin-embedded Human prostate tissue using anti-FCGR2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fc-gamma-RIIA-CD32a-Antibody-9G5-Immunohistochemistry-Paraffin-NBP1-47735-img0021.jpg "Immunohistochemistry-Paraffin: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Staining of paraffin-embedded Human prostate tissue using anti-FCGR2A mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FCGR2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fc-gamma-RIIA-CD32a-Antibody-9G5-Immunohistochemistry-Paraffin-NBP1-47735-img0014.jpg "Immunohistochemistry-Paraffin: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FCGR2A mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Staining of paraffin-embedded Human breast tissue using anti-FCGR2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fc-gamma-RIIA-CD32a-Antibody-9G5-Immunohistochemistry-Paraffin-NBP1-47735-img0015.jpg "Immunohistochemistry-Paraffin: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Staining of paraffin-embedded Human breast tissue using anti-FCGR2A mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Staining of paraffin-embedded Human colon tissue using anti-FCGR2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fc-gamma-RIIA-CD32a-Antibody-9G5-Immunohistochemistry-Paraffin-NBP1-47735-img0016.jpg "Immunohistochemistry-Paraffin: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Staining of paraffin-embedded Human colon tissue using anti-FCGR2A mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Staining of paraffin-embedded Human endometrium tissue using anti-FCGR2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fc-gamma-RIIA-CD32a-Antibody-9G5-Immunohistochemistry-Paraffin-NBP1-47735-img0017.jpg "Immunohistochemistry-Paraffin: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Staining of paraffin-embedded Human endometrium tissue using anti-FCGR2A mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Staining of paraffin-embedded Human Kidney tissue using anti-FCGR2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fc-gamma-RIIA-CD32a-Antibody-9G5-Immunohistochemistry-Paraffin-NBP1-47735-img0018.jpg "Immunohistochemistry-Paraffin: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Staining of paraffin-embedded Human Kidney tissue using anti-FCGR2A mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Staining of paraffin-embedded Human lung tissue using anti-FCGR2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fc-gamma-RIIA-CD32a-Antibody-9G5-Immunohistochemistry-Paraffin-NBP1-47735-img0019.jpg "Immunohistochemistry-Paraffin: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Staining of paraffin-embedded Human lung tissue using anti-FCGR2A mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Staining of paraffin-embedded Human pancreas tissue using anti-FCGR2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fc-gamma-RIIA-CD32a-Antibody-9G5-Immunohistochemistry-Paraffin-NBP1-47735-img0020.jpg "Immunohistochemistry-Paraffin: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Staining of paraffin-embedded Human pancreas tissue using anti-FCGR2A mouse monoclonal antibody.")
![Immunoprecipitation: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Lane 1: Negative Control Lane 2: NBP1-47738 Lane 3: NBP1-47739 Lane 4: NBP1-47740 Lane 5: NBP1-47741 Lane 6: NBP1-47742 Lane 7: NBP1-47734 Lane 8: NBP1-47735 Lane 9: NBP1-47736 Lane 10: NBP1-47737 Lane 11: NBP1-47743 Lane 12: NBP1-47744](http://images.novusbio.com/fullsize/Fc-gamma-RIIA-CD32a-Antibody-9G5-Immunoprecipitation-NBP1-47735-img0023.jpg "Immunoprecipitation: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Lane 1: Negative Control Lane 2: NBP1-47738 Lane 3: NBP1-47739 Lane 4: NBP1-47740 Lane 5: NBP1-47741 Lane 6: NBP1-47742 Lane 7: NBP1-47734 Lane 8: NBP1-47735 Lane 9: NBP1-47736 Lane 10: NBP1-47737 Lane 11: NBP1-47743 Lane 12: NBP1-47744")
![SDS-PAGE Fc gamma RIIIA/CD16a [Unconjugated]](https://images.novusbio.com/images2/Fc_gamma_RIIIA_4325-FC_411.jpg)
![Binding Activity Fc gamma RIIIA/CD16a [Unconjugated]](https://images.novusbio.com/images2/Fc_gamma_RIIIA_4325-FC_491.jpg)
![SEC-MALS Fc gamma RIIIA/CD16a [Unconjugated]](https://images.novusbio.com/images/4325-fc_recombinant-human-fc-gamma-riiia-cd16a-protein-cf-sec-mals-2842023172947.jpg)
![Western Blot Fc gamma RIII (CD16) Antibody [Unconjugated]](https://images.novusbio.com/images2/Fc_gamma_RIII_AF1597_Western_Blot_23213.jpg)
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![Binding Activity Fc gamma RI/CD64 [Unconjugated]](https://images.novusbio.com/images2/Fc_gamma_RI_1257FC_2060.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Bioactivity IL-4 [Unconjugated]](https://images.novusbio.com/images/protein/6507-ilcf_recombinant-human-il-4-cho-expressed-protein-cf-bioactivity-272020133214.jpg)
![Immunocytochemistry Integrin beta 2/CD18 Antibody [Unconjugated]](https://images.novusbio.com/images2/Integrin_beta_2_AF1730_Immunocytochemistry_6474.jpg)
![Immunocytochemistry Integrin beta 2/CD18 Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af1730_human-integrin-beta-2-cd18-affinity-purified-polyclonal-ab-immunocytochemistry-1292023225343.jpg)
![N/A CD14 [HRP]](https://images.novusbio.com/images2/CD14_DC140_ELISA_42.jpg)
![N/A CD14 [HRP]](https://images.novusbio.com/images2/DATA_CD14_DC140_ELISA_597.jpg)
![N/A C-Reactive Protein/CRP [Biotin]](https://images.novusbio.com/images2/DATA_CReactive_Protein_DY1707_ELISA_1701.jpg)
![Immunohistochemistry ICAM-1/CD54 Antibody [Unconjugated]](https://images.novusbio.com/images2/ICAM1_AF796_Immunohistochemistry_17354.jpg)
![Western Blot ICAM-1/CD54 Antibody [Unconjugated]](https://images.novusbio.com/images/af796_mouse-icam-1-cd54-affinity-purified-polyclonal-ab-4120241048112.jpg)
![Western Blot ICAM-1/CD54 Antibody [Unconjugated]](https://images.novusbio.com/images/af796_mouse-icam-1-cd54-affinity-purified-polyclonal-ab-41202410481112.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![N/A IL-10 [Biotin]](https://images.novusbio.com/images2/DATA_IL10_DY417_ELISA_2014.jpg)
![SDS-PAGE Fc gamma RIIB/C (CD32b/c) [Unconjugated]](https://images.novusbio.com/images/protein/Fc_gamma_RIIB_C_-CD32b_c-_1875-CD_632.jpg)
![Binding Activity Fc gamma RIIB/C (CD32b/c) [Unconjugated]](https://images.novusbio.com/images/protein/Fc_gamma_RIIB_C_-CD32b_c-_1875-CD_642.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")