![Immunohistochemistry: ERCC1 Antibody (8F1) - Azide and BSA Free [NBP2-80725] - Analysis of ERCC1 in human non-small cell lung cancer. Image courtesy of product review submitted by Alex Soltermann. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/ERCC1-Antibody-8F1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80725-img0001.jpg "Immunohistochemistry: ERCC1 Antibody (8F1) - Azide and BSA Free [NBP2-80725] - Analysis of ERCC1 in human non-small cell lung cancer. Image courtesy of product review submitted by Alex Soltermann. Image from the standard format of this antibody.")
| Reactivity | Hu, Rt, ChHaSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | 8F1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Full length recombinant human ERCC1. [UniProt# P07992] |
| Localization | Nuclear. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ERCC1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IHC: In an ABC method, we suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | ERCC1 |
![Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) - Azide and BSA Free [NBP2-80725] - Formalin fixed paraffin embedded human tonsil stained with ERCC1 antibody. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/ERCC1-Antibody-8F1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80725-img0002.jpg "Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) - Azide and BSA Free [NBP2-80725] - Formalin fixed paraffin embedded human tonsil stained with ERCC1 antibody. Image from the standard format of this antibody.")
