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eEF-2 Antibody

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Biological Strategies: Western Blot: eEF-2 Antibody [NB100-87020] - Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibody used at 0.04 ug/ml.
Immunohistochemistry-Paraffin: eEF-2 Antibody [NB100-87020] - Section of human breast carcinoma (left) and mouse teratoma (right).Antibody: Affinity purified rabbit anti-eEF2 used at a dilution of 1:1,000 (0.2ug/ml). ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, PmSpecies Glossary
Applications WB, IHC, IP (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
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Biological Strategies

     

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eEF-2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 808 and 858 of human eukaryotic translation elongation factor 2 using the numbering given in entry NP_001952.1 (GeneID 1938).
Predicted Species
Rat (100%), Chicken (100%), Primate (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EEF2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:2000-1:10000
Application Notes
This antibody is not recommended for immunoprecipitation.

Reactivity Notes

Orangutan (100%).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for eEF-2 Antibody

  • eEF2
  • eEF-2
  • EF-2
  • EF2EEF-2
  • elongation factor 2
  • eukaryotic translation elongation factor 2
  • polypeptidyl-tRNA translocase

Background

This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EEF2
Entrez
Uniprot