![Western Blot: DYKDDDDK Epitope Tag Antibody (FG4R) [NBP2-37823] - Analysis using [NBP2-37823] at a 1:1000 (1ug/mL) dilution, probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector; untransfected (1) and transfected (2). Observed molecular weight ~130 kDa.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-FG4R-Western-Blot-NBP2-37823-img0011.jpg "Western Blot: DYKDDDDK Epitope Tag Antibody (FG4R) [NBP2-37823] - Analysis using [NBP2-37823] at a 1:1000 (1ug/mL) dilution, probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector; untransfected (1) and transfected (2). Observed molecular weight ~130 kDa.")
| Reactivity | ETSpecies Glossary |
| Applications | WB, ICC/IF, IP |
| Clone | FG4R |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | DYKDDDDK Epitope Tag Antibody (FG4R) was made to a Synthetic peptide (DYKDDDDK) coupled to KLH. |
| Specificity | DYKDDDDK Epitope Tag Antibody (FG4R) detects Flag tag from various samples. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 1.01 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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Hoist the DYKDDDDK Epitope Tag The DYKDDDDK Epitope Tag antibody was raised against a short, artificial sequence that is widely and commonly used as a protein fusion tag. It is pervasively present in a vast amount of scientific studies, and is a very powerful investigative research... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody (FG4R) [NBP2-37823] - Analysis of Chlamydia trachomatis FLAG-tagged IncA (blue) in HeLa cells. Cells were infected with a GFP-expressing strain of Chlamydia trachomatis containing FLAG-IncA under the control of the Tetracycline (Tet) promoter, and either left untreated (0ng/ml Tet) or treated with anhydrotetracycline (10ng/ml Tet). Cells were fixed with 2% paraformaldehyde in PBS for 30 min at RT, permeabilized and blocked in 10% goat serum, 0.1% BSA, 0.1% saponin in PBS pH 7.4 for 30 mins at RT, then probed with a DYKDDDDK Epitope Tag Antibody (FG4R) [NBP2-37823] at 1:500 for 1 hr at RT. Cells were washed with PBS, and incubated with an AlexaFluor 647-conjugated goat anti-mouse IgG secondary antibody at 1:2000 for 1 hr at RT. Images were taken on a fluorescent microscope using a 60X oil-immersion objective. Scale bars represent 20um. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-FG4R-Immunocytochemistry-Immunofluorescence-NBP2-37823-img0009.jpg "Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody (FG4R) [NBP2-37823] - Analysis of Chlamydia trachomatis FLAG-tagged IncA (blue) in HeLa cells. Cells were infected with a GFP-expressing strain of Chlamydia trachomatis containing FLAG-IncA under the control of the Tetracycline (Tet) promoter, and either left untreated (0ng/ml Tet) or treated with anhydrotetracycline (10ng/ml Tet). Cells were fixed with 2% paraformaldehyde in PBS for 30 min at RT, permeabilized and blocked in 10% goat serum, 0.1% BSA, 0.1% saponin in PBS pH 7.4 for 30 mins at RT, then probed with a DYKDDDDK Epitope Tag Antibody (FG4R) [NBP2-37823] at 1:500 for 1 hr at RT. Cells were washed with PBS, and incubated with an AlexaFluor 647-conjugated goat anti-mouse IgG secondary antibody at 1:2000 for 1 hr at RT. Images were taken on a fluorescent microscope using a 60X oil-immersion objective. Scale bars represent 20um. Data courtesy of the Innovators Program.")
![Western Blot: DYKDDDDK Epitope Tag Antibody (FG4R) [NBP2-37823] - Analysis of 30ug of whole cell lysates from HeLa cells infected with a strain of Chlamydia trachomatis expressing FLAG-IncA under the control of a Tetracycline promoter (Tet), and either left untreated (-) or treated with anhydrotetracycline (+), onto an SDS-PAGE gel, using [NBP2-37823]. Observed molecular weight ~30 kDa.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-FG4R-Western-Blot-NBP2-37823-img0012.jpg "Western Blot: DYKDDDDK Epitope Tag Antibody (FG4R) [NBP2-37823] - Analysis of 30ug of whole cell lysates from HeLa cells infected with a strain of Chlamydia trachomatis expressing FLAG-IncA under the control of a Tetracycline promoter (Tet), and either left untreated (-) or treated with anhydrotetracycline (+), onto an SDS-PAGE gel, using [NBP2-37823]. Observed molecular weight ~30 kDa.")
![Immunoprecipitation: DYKDDDDK Epitope Tag Antibody (FG4R) [NBP2-37823] - Analysis of Chlamydia trachomatis FLAG-tagged IncA in HeLa cells infected with a strain of Chlamydia trachomatis expressing FLAG-IncA under control of a Tetracycline (Tet) promoter, untreated (-) or treated with anhydrotetracycline (+). Complexes formed by incubating 1ml of HeLa cell lysate with 3ug of [NBP2-37823] overnight at 4C. Immune complexes were captured on 20ul Protein G agarose and eluted by boiling in Laemmli sample buffer. Samples were resolved on a 10% Bis-Tris acrylamide gel, transferred to a PVDF membrane, and blocked with 5% milk in TBST for 1 hr at RT. Membrane was probed with a rabbit anti-FLAG pAb at 1:200 in 3% BSA in TBST overnight at 4C, washed in TBST, and incubated with an HRP-conjugated donkey anti-rabbit IgG secondary Ab at 1:15000 in 1% milk in TBST for 1 hr at RT. Chemiluminescence performed using SuperSignal West Dura. Data courtesy of Innovators Program.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-FG4R-Immunocytochemistry-Immunofluorescence-NBP2-37823-img0010.jpg "Immunoprecipitation: DYKDDDDK Epitope Tag Antibody (FG4R) [NBP2-37823] - Analysis of Chlamydia trachomatis FLAG-tagged IncA in HeLa cells infected with a strain of Chlamydia trachomatis expressing FLAG-IncA under control of a Tetracycline (Tet) promoter, untreated (-) or treated with anhydrotetracycline (+). Complexes formed by incubating 1ml of HeLa cell lysate with 3ug of [NBP2-37823] overnight at 4C. Immune complexes were captured on 20ul Protein G agarose and eluted by boiling in Laemmli sample buffer. Samples were resolved on a 10% Bis-Tris acrylamide gel, transferred to a PVDF membrane, and blocked with 5% milk in TBST for 1 hr at RT. Membrane was probed with a rabbit anti-FLAG pAb at 1:200 in 3% BSA in TBST overnight at 4C, washed in TBST, and incubated with an HRP-conjugated donkey anti-rabbit IgG secondary Ab at 1:15000 in 1% milk in TBST for 1 hr at RT. Chemiluminescence performed using SuperSignal West Dura. Data courtesy of Innovators Program.")
