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Human Dopamine D1R/DRD1 ELISA Kit (Colorimetric)

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ELISA: Human Dopamine D1R/DRD1 ELISA Kit (Colorimetric) [NBP2-67263] - Samples were spiked with high concentrations of Human Dopamine D1R/DRD1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human Dopamine D1R/DRD1 ELISA Kit (Colorimetric) [NBP2-67263] - Samples were spiked with high concentrations of Human Dopamine D1R/DRD1 and diluted with Reference Standard & Sample Diluent to produce samples with ...read more
ELISA: Human Dopamine D1R/DRD1 ELISA Kit (Colorimetric) [NBP2-67263] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL

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Human Dopamine D1R/DRD1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human D1R/DRD1 in samples. No significant cross-reactivity or interference between Human D1R/DRD1 and analogues was observed.
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.93%
Intra-Assay
CV% < 4.73%
Spike Recovery
85-108%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
DRD1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Dopamine D1R/DRD1 ELISA Kit (Colorimetric)

  • D(1A) dopamine receptor
  • D1DR
  • DADR
  • Dopamine D1 R
  • Dopamine D1 receptor
  • Dopamine D1R
  • dopamine receptor D1
  • DRD1
  • DRD1A

Background

The members of the G protein-coupled receptor family are distinguished by their slow transmitting response to ligand binding. These seven transmembrane proteins include the adrenergic, serotonin, and dopamine receptors. The effect of the signaling molecule can be excitatory or inhibitory depending on the type of receptor to which it binds. Beta-adrenergic receptor bound to adrenaline activates adenylyl cyclase, while alpha 2-adrenergic receptor bound to adrenaline inhibits adenylyl cyclase. The dopamine receptors are divided into two classes; D1 and D2, which differ in their functional characteristics. D1 receptors stimulate adenylyl cyclase, while D2 receptors inhibit adenylyl cyclase activity. Five different subtypes of dopamine receptor have been described to date. D1DR and D5DR belong to the D1 subclass, while D2DR, D3DR and D4DR belong to the D2 subclass of dopamine receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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  Human Dopamine D1R/DRD1 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol DRD1