![Western Blot: Dopa Decarboxylase/DDC Antibody [NB300-252] - Analysis of DOPA Decarboxylase, Human in bovine adrenal medulla lysate using this antibody at 1:1000. Showing specific immunolabeling of the ~55k DOPA decarboxylase protein.](http://images.novusbio.com/fullsize/Dopa-Decarboxylase-DDC-Antibody-Western-Blot-NB300-252-img0005.jpg "Western Blot: Dopa Decarboxylase/DDC Antibody [NB300-252] - Analysis of DOPA Decarboxylase, Human in bovine adrenal medulla lysate using this antibody at 1:1000. Showing specific immunolabeling of the ~55k DOPA decarboxylase protein.")
| Reactivity | Hu, Rt, Bv, Ca, Gp, Rb, ShSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH. Accession # P20711 |
| Specificity | Specific for endogenous levels of the ~55 kDa Dopa Decarboxylase/DDC protein. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DDC |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Recognizes DDC in human adrenal medulla and basal ganglia. Use in Immunohistochemistry and Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 28398344). |
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| Theoretical MW | 55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Preservative | No Preservative |
| Purity | Antigen Affinity-purified |
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| Publication using NB300-252 | Applications | Species |
|---|---|---|
| Rivetti di Val Cervo P, Romanov RA, Spigolon G et al. Induction of functional dopamine neurons from human astrocytes in vitro and mouse astrocytes in a Parkinson's disease model. Nat. Biotechnol. 2017-04-10 [PMID: 28398344] (ICC/IF, IF/IHC) | ICC/IF, IF/IHC |
Secondary Antibodies |
Isotype Controls |
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