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Dkk-1 Antibody (2A5)

 (11 Publications)
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Western Blot: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of DKK1 expression in U-2 OS (Cat # L022V1).
Western Blot: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of DKK1 expression in U-2 OS (Cat # L022V1).
Immunohistochemistry-Paraffin: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of monoclonal antibody to DKK1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml
Immunoprecipitation: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of DKK1 transfected lysate using anti-DKK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with DKK1 rabbit polyclonal antibody.
ELISA: Dkk-1 Antibody (2A5) [H00022943-M11] - Detection limit for recombinant GST tagged DKK1 is 0.1 ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, YeSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IP, MA
Clone
2A5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Dkk-1 Antibody (2A5) Summary

Immunogen
DKK1 (AAH01539.1, 1 a.a. ~ 266 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MMALGAAGATRVSVAMVAAALGGHPLLGVSATLNSVLNSNAIKNLPPPLGGAAGHPGSAVSAAPGILYPGGNKYQTIDNYQPYPCAEDEECGTDEYCASPTRGGDAGVQICLACRKRRKRCMRHAMCCPGNYCKNGICVSSDQNHFRGEIEETITESFGNDHSTLDGYSRRTTLSSKMYHTTGQEGSVCLRSSDCASGLCCARHFWSKICKPVLKGGQVCTKHRRKGSHGLEIFQRCYCGEGLSCRIQKDHHQASNSSRLHTCQRH
Specificity
DKK1 - dickkopf homolog 1 (Xenopus laevis)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DKK1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Microarray
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA. Immunocytochemistry/Immunofluorescence was reported in scientific literature.
Publications
Read Publications using
H00022943-M11 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Dkk-1 Antibody (2A5)

  • dickkopf (Xenopus laevis) homolog 1
  • dickkopf homolog 1 (Xenopus laevis)
  • dickkopf related protein-1
  • Dickkopf-1
  • dickkopf-related protein 1
  • Dkk1
  • Dkk-1
  • hDkk-1
  • SKdickkopf-1 like

Background

This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Dkk-1 Antibody
(2A5)
H00022943-M11
Dkk-1 Antibody (2A5)
Species: Hu, Ye
Applications: WB, ELISA, ICC/IF, IHC, IP, MA

Publications for Dkk-1 Antibody (H00022943-M11)(11)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(1)
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Human
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using H00022943-M11 Applications Species
Joo K, Sung-Hye P, Seung C et al. Molecular subgrouping of medulloblastoma in pediatric population using the NanoString assay and comparison with immunohistochemistry methods. BMC Cancer. 2022-11-28 [PMID: 36437460]
T Suda, T Yamashita, H Sunagozaka, H Okada, K Nio, Y Sakai, T Yamashita, E Mizukoshi, M Honda, S Kaneko Dickkopf-1 Promotes Angiogenesis and is a Biomarker for Hepatic Stem Cell-like Hepatocellular Carcinoma International Journal of Molecular Sciences, 2022-03-03;23(5):. 2022-03-03 [PMID: 35269944]
Robson JP, Remke M, Kool M et al. Identification of CD24 as a marker of Patched1 deleted medulloblastoma-initiating neural progenitor cells. PLoS One 2019-04-05 [PMID: 30657775]
Shinojima N, Nakamura H, Tasaki M et al. A patient with medulloblastoma in its early developmental stage. J Neurosurg Pediatr 2014-12-01 [PMID: 25303160]
Yi N, Liao QP, Li ZH et al. RNA interference-mediated targeting of DKK1 gene expression in Ishikawa endometrial carcinoma cells causes increased tumor cell invasion and migration. ONCOLOGY LETTERS. 2013-01-01 [PMID: 24137406]
Min HS, Lee JY, Kim SK, Park SH. Genetic grouping of medulloblastomas by representative markers in pathologic diagnosis. Transl Oncol. 2013-06-01 [PMID: 23730405]
Shi RY, Yang XR, Shen QJ et al. High expression of Dickkopf-related protein 1 is related to lymphatic metastasis and indicates poor prognosis in intrahepatic cholangiocarcinoma patients after surgery Cancer 2012-11-06 [PMID: 23132676] (ICC/IF, WB, Human) ICC/IF, WB Human
Taylor MD, Northcott PA, Korshunov A et al. Molecular subgroups of medulloblastoma: the current consensus. Acta Neuropathol 2012-04-01 [PMID: 22134537]
Macdonald LJ, Sales KJ, Grant V et al. Prokineticin 1 induces Dickkopf 1 expression and regulates cell proliferation and decidualization in the human endometrium. Mol Hum Reprod. 2011-05-05 [PMID: 21546446]
Shin HJ, Rho SB, Jung DC et al. Carbonic anhydrase IX (CA9) modulates tumor-associated cell migration and invasion. J Cell Sci. 2011-03-01 [PMID: 21363891]
Show All 11 Publications.

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Bioinformatics

Gene Symbol DKK1
Entrez
OMIM
Uniprot