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DFF40/CAD Antibody (36A349) [Janelia Fluor® 669]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clone
36A349
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 669

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB120-13592AF350
Alexa Fluor 405
NB120-13592AF405
Alexa Fluor 488
NB120-13592AF488
Alexa Fluor 532
NB120-13592AF532
Alexa Fluor 594
NB120-13592AF594
Alexa Fluor 647
NB120-13592AF647
Alexa Fluor 700
NB120-13592AF700
Alexa Fluor 750
NB120-13592AF750
Biotin
NB120-13592B
CoraFluor 1
NB120-13592CL1
DyLight 350
NB120-13592UV
DyLight 405
NB120-13592V
DyLight 488
NB120-13592G
DyLight 550
NB120-13592R
DyLight 594
NB120-13592DL594
DyLight 650
NB120-13592C
DyLight 680
NB120-13592FR
DyLight 755
NB120-13592IR
FITC
NB120-13592F
HRP
NB120-13592H
Janelia Fluor 525
NB120-13592JF525
Janelia Fluor 549
NB120-13592JF549
Janelia Fluor 635
NB120-13592JF635
Janelia Fluor 646
NB120-13592JF646
Unconjugated
NB120-13592
mFluor Violet 450 SE
NB120-13592MFV450
mFluor Violet 500 SE
NB120-13592MFV500
mFluor Violet 610 SE
NB120-13592MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB120-13592
BSA Free
NB120-13592SS
BSA Free
NB120-13592
Azide and BSA Free
NBP2-80696
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DFF40/CAD Antibody (36A349) [Janelia Fluor® 669] Summary

Immunogen
Anti-DFF40 monoclonal antibody was raised against a peptide corresponding to amino acids 205 to 222 of human DFF40.
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
DFFB
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24051182).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for DFF40/CAD Antibody (36A349) [Janelia Fluor® 669]

  • CAD
  • CADCaspase-activated nuclease
  • Caspase-activated deoxyribonuclease
  • Caspase-activated DNase
  • CPAN
  • CPANDNA fragmentation factor 40 kDa subunit
  • DFF2
  • DFF40
  • DFF-40DFF40DFF2DNA fragmentation factor subunit beta
  • DFFB
  • DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
  • EC 3.-

Background

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40. Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively (1, 2, 5). Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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DFF40/CAD Antibody
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DFF40/CAD Antibody (36A349) [Janelia Fluor® 669]
Species: Hu, Mu
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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for DFF40/CAD Antibody (NB120-13592JF669). (Showing 1 - 1 of 1 FAQ).

  1. Does Novus offer any CAD (caspase activated DNase) antibodies that specifically look at the activation state of CAD? And if so, how well does this antibody work with chicken?
    • CAD/DFF40/DFFB is inhibited by being bound by DFFA. Because of this manner of inhibition, antibodies will not be able to recognize the difference between activated and inhibited DFFB, so we do not have a product specifically for activated or inhibited DFFB and this product will probably be nearly impossible to make. Unfortunately, the human and chicken sequence appear to be divergent for any of our immunogens to recommend any antibody to test for cross-reactivity with chicken. I apologize for the inconvenience.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol DFFB