![Western Blot: Cytokeratin 71 Antibody [NBP2-17040] - Cytokeratin 71 Sample (30 ug of whole cell lysate) A: HepG2 7. 5% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/Cytokeratin-71-Antibody-Western-Blot-NBP2-17040-img0003.jpg "Western Blot: Cytokeratin 71 Antibody [NBP2-17040] - Cytokeratin 71 Sample (30 ug of whole cell lysate) A: HepG2 7. 5% SDS PAGE gel, diluted at 1:5000.")
| Reactivity | Hu, Sh, Rt, Bv, Ca, FeSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cytokeratin 71. The exact sequence is proprietary. |
| Predicted Species | Rat (91%), Canine (91%), Bovine (91%), Feline (93%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | KRT71 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 57 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris-Glycine (pH7), 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
| Publication using NBP2-17040 | Applications | Species |
|---|---|---|
| Montandon Sophie A, Tzika Athanasia C, Martins Antonio F, et al. Two waves of anisotropic growth generate enlarged follicles in the spiny mouse. Evodevo. 2014-09-25 [PMID: 25705371] (Mouse) | Mouse |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040] - Densities of proliferating and apoptotic cells in a spine follicle. Longitudinal sections of a 28-day-old Acomys. pH3 (green), K71 (red), and Hoechst (blue) immunostaining. Image collected and cropped by CiteAb from the following publication (evodevojournal.biomedcentral.com/articles/10.1186/2041-9139-5-33), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cytokeratin-71-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-17040-img0008.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040] - Densities of proliferating and apoptotic cells in a spine follicle. Longitudinal sections of a 28-day-old Acomys. pH3 (green), K71 (red), and Hoechst (blue) immunostaining. Image collected and cropped by CiteAb from the following publication (evodevojournal.biomedcentral.com/articles/10.1186/2041-9139-5-33), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Cytokeratin 71 Antibody [NBP2-17040] - Colon carcinoma. KRT71 antibody [N3C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Cytokeratin-71-Antibody-Immunohistochemistry-Paraffin-NBP2-17040-img0002.jpg "Immunohistochemistry-Paraffin: Cytokeratin 71 Antibody [NBP2-17040] - Colon carcinoma. KRT71 antibody [N3C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KRT71 protein stained by KRT71 antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Cytokeratin-71-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-17040-img0005.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KRT71 protein stained by KRT71 antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040] - Observed and simulated cell proliferation and cell death along a spine follicle. Transverse sections (28-day-old Acomys) are taken along the spine long axis, from the basis of the follicle (rows A-C), to the DP-medulla transition (rows D-E), to the collapse of the keratinized medulla (rows F-G). H&E, Hematoxylin and Eosin staining. Immunostaining: Kpan, Pan-Keratin (red); Lmn, Laminin (green); BrdU, 5-bromo-2'-deoxyuridine (green); K71, keratin 71 (red); pH3, phospho-Histone H3 (green); TUNEL (red), Hoechst (blue). Arrows: IRS, DP: dermal papilla, Mx: matrix, C: cortex, M: medulla. Simulated transverse sections (Sim) are snapshots of the Supplementary Movie 2 showing time evolution of a simulated follicle. Scale bars: 50 um. Image collected and cropped by CiteAb from the following publication (evodevojournal.biomedcentral.com/articles/10.1186/2041-9139-5-33), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cytokeratin-71-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-17040-img0006.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040] - Observed and simulated cell proliferation and cell death along a spine follicle. Transverse sections (28-day-old Acomys) are taken along the spine long axis, from the basis of the follicle (rows A-C), to the DP-medulla transition (rows D-E), to the collapse of the keratinized medulla (rows F-G). H&E, Hematoxylin and Eosin staining. Immunostaining: Kpan, Pan-Keratin (red); Lmn, Laminin (green); BrdU, 5-bromo-2'-deoxyuridine (green); K71, keratin 71 (red); pH3, phospho-Histone H3 (green); TUNEL (red), Hoechst (blue). Arrows: IRS, DP: dermal papilla, Mx: matrix, C: cortex, M: medulla. Simulated transverse sections (Sim) are snapshots of the Supplementary Movie 2 showing time evolution of a simulated follicle. Scale bars: 50 um. Image collected and cropped by CiteAb from the following publication (evodevojournal.biomedcentral.com/articles/10.1186/2041-9139-5-33), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040] - Densities of proliferating and apoptotic cells in a spine follicle. Longitudinal sections of a 28-day-old Acomys. BrdU (green), K71 (red), and Hoechst (blue) immunostaining. Image collected and cropped by CiteAb from the following publication (evodevojournal.biomedcentral.com/articles/10.1186/2041-9139-5-33), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cytokeratin-71-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-17040-img0007.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040] - Densities of proliferating and apoptotic cells in a spine follicle. Longitudinal sections of a 28-day-old Acomys. BrdU (green), K71 (red), and Hoechst (blue) immunostaining. Image collected and cropped by CiteAb from the following publication (evodevojournal.biomedcentral.com/articles/10.1186/2041-9139-5-33), licensed under a CC-BY license.")
![Immunocytochemistry/ Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040] - Densities of proliferating & apoptotic cells in a spine follicle. Longitudinal sections of a 28-day-old Acomys.(A) pH3 (green), K71 (red), & Hoechst (blue) immunostaining. (B) BrdU (green), K71 (red), & Hoechst (blue) immunostaining. (C) BrdU (green), TUNEL (red), & Hoechst (blue) immunostaining. (D–F) Color-coded local relative densities in a radius that corresponds to 3% of the image diagonal (blue: low density, red: high density) of cells labelled with Hoechst (D), BrdU (E), & TUNEL (F). Scale bars: 100 μm. Image collected & cropped by CiteAb from the following publication (//evodevojournal.biomedcentral.com/articles/10.1186/2041-9139-5-33), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-17040_rabbit-polyclonal-cytokeratin-71-antibody-31020241529338.jpg "Immunocytochemistry/ Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040] - Densities of proliferating & apoptotic cells in a spine follicle. Longitudinal sections of a 28-day-old Acomys.(A) pH3 (green), K71 (red), & Hoechst (blue) immunostaining. (B) BrdU (green), K71 (red), & Hoechst (blue) immunostaining. (C) BrdU (green), TUNEL (red), & Hoechst (blue) immunostaining. (D–F) Color-coded local relative densities in a radius that corresponds to 3% of the image diagonal (blue: low density, red: high density) of cells labelled with Hoechst (D), BrdU (E), & TUNEL (F). Scale bars: 100 μm. Image collected & cropped by CiteAb from the following publication (//evodevojournal.biomedcentral.com/articles/10.1186/2041-9139-5-33), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040] - Observed & simulated cell proliferation & cell death along a spine follicle. Transverse sections (28-day-old Acomys) are taken along the spine long axis, from the basis of the follicle (rows A-C), to the DP-medulla transition (rows D-E), to the collapse of the keratinized medulla (rows F-G). H&E, Hematoxylin & Eosin staining. Immunostaining: Kpan, Pan-Keratin (red); Lmn, Laminin (green); BrdU, 5-bromo-2'-deoxyuridine (green); K71, keratin 71 (red); pH3, phospho-Histone H3 (green); TUNEL (red), Hoechst (blue). Arrows: IRS, DP: dermal papilla, Mx: matrix, C: cortex, M: medulla. Simulated transverse sections (Sim) are snapshots of the Supplementary Movie 2 showing time evolution of a simulated follicle. Scale bars: 50 μm. Image collected & cropped by CiteAb from the following publication (//evodevojournal.biomedcentral.com/articles/10.1186/2041-9139-5-33), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-17040_rabbit-polyclonal-cytokeratin-71-antibody-310202416114784.jpg "Immunocytochemistry/ Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040] - Observed & simulated cell proliferation & cell death along a spine follicle. Transverse sections (28-day-old Acomys) are taken along the spine long axis, from the basis of the follicle (rows A-C), to the DP-medulla transition (rows D-E), to the collapse of the keratinized medulla (rows F-G). H&E, Hematoxylin & Eosin staining. Immunostaining: Kpan, Pan-Keratin (red); Lmn, Laminin (green); BrdU, 5-bromo-2'-deoxyuridine (green); K71, keratin 71 (red); pH3, phospho-Histone H3 (green); TUNEL (red), Hoechst (blue). Arrows: IRS, DP: dermal papilla, Mx: matrix, C: cortex, M: medulla. Simulated transverse sections (Sim) are snapshots of the Supplementary Movie 2 showing time evolution of a simulated follicle. Scale bars: 50 μm. Image collected & cropped by CiteAb from the following publication (//evodevojournal.biomedcentral.com/articles/10.1186/2041-9139-5-33), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040] - Observed & simulated cell proliferation & cell death along a spine follicle. Transverse sections (28-day-old Acomys) are taken along the spine long axis, from the basis of the follicle (rows A-C), to the DP-medulla transition (rows D-E), to the collapse of the keratinized medulla (rows F-G). H&E, Hematoxylin & Eosin staining. Immunostaining: Kpan, Pan-Keratin (red); Lmn, Laminin (green); BrdU, 5-bromo-2'-deoxyuridine (green); K71, keratin 71 (red); pH3, phospho-Histone H3 (green); TUNEL (red), Hoechst (blue). Arrows: IRS, DP: dermal papilla, Mx: matrix, C: cortex, M: medulla. Simulated transverse sections (Sim) are snapshots of the Supplementary Movie 2 showing time evolution of a simulated follicle. Scale bars: 50 μm. Image collected & cropped by CiteAb from the following publication (//evodevojournal.biomedcentral.com/articles/10.1186/2041-9139-5-33), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-17040_rabbit-polyclonal-cytokeratin-71-antibody-310202416114728.jpg "Immunocytochemistry/ Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040] - Observed & simulated cell proliferation & cell death along a spine follicle. Transverse sections (28-day-old Acomys) are taken along the spine long axis, from the basis of the follicle (rows A-C), to the DP-medulla transition (rows D-E), to the collapse of the keratinized medulla (rows F-G). H&E, Hematoxylin & Eosin staining. Immunostaining: Kpan, Pan-Keratin (red); Lmn, Laminin (green); BrdU, 5-bromo-2'-deoxyuridine (green); K71, keratin 71 (red); pH3, phospho-Histone H3 (green); TUNEL (red), Hoechst (blue). Arrows: IRS, DP: dermal papilla, Mx: matrix, C: cortex, M: medulla. Simulated transverse sections (Sim) are snapshots of the Supplementary Movie 2 showing time evolution of a simulated follicle. Scale bars: 50 μm. Image collected & cropped by CiteAb from the following publication (//evodevojournal.biomedcentral.com/articles/10.1186/2041-9139-5-33), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040] - Observed & simulated cell proliferation & cell death along a spine follicle. Transverse sections (28-day-old Acomys) are taken along the spine long axis, from the basis of the follicle (rows A-C), to the DP-medulla transition (rows D-E), to the collapse of the keratinized medulla (rows F-G). H&E, Hematoxylin & Eosin staining. Immunostaining: Kpan, Pan-Keratin (red); Lmn, Laminin (green); BrdU, 5-bromo-2'-deoxyuridine (green); K71, keratin 71 (red); pH3, phospho-Histone H3 (green); TUNEL (red), Hoechst (blue). Arrows: IRS, DP: dermal papilla, Mx: matrix, C: cortex, M: medulla. Simulated transverse sections (Sim) are snapshots of the Supplementary Movie 2 showing time evolution of a simulated follicle. Scale bars: 50 μm. Image collected & cropped by CiteAb from the following publication (//evodevojournal.biomedcentral.com/articles/10.1186/2041-9139-5-33), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-17040_rabbit-polyclonal-cytokeratin-71-antibody-31020241613649.jpg "Immunocytochemistry/ Immunofluorescence: Cytokeratin 71 Antibody [NBP2-17040] - Observed & simulated cell proliferation & cell death along a spine follicle. Transverse sections (28-day-old Acomys) are taken along the spine long axis, from the basis of the follicle (rows A-C), to the DP-medulla transition (rows D-E), to the collapse of the keratinized medulla (rows F-G). H&E, Hematoxylin & Eosin staining. Immunostaining: Kpan, Pan-Keratin (red); Lmn, Laminin (green); BrdU, 5-bromo-2'-deoxyuridine (green); K71, keratin 71 (red); pH3, phospho-Histone H3 (green); TUNEL (red), Hoechst (blue). Arrows: IRS, DP: dermal papilla, Mx: matrix, C: cortex, M: medulla. Simulated transverse sections (Sim) are snapshots of the Supplementary Movie 2 showing time evolution of a simulated follicle. Scale bars: 50 μm. Image collected & cropped by CiteAb from the following publication (//evodevojournal.biomedcentral.com/articles/10.1186/2041-9139-5-33), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")