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Cystatin SN Overexpression Lysate

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Western Blot: Cystatin SN Overexpression Lysate (Adult Normal) [NBP2-10786] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cystatin SN.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

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Cystatin SN Overexpression Lysate Summary

Description

Cystatin SN Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC202896

Accession#: NM_001898

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
CST1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
14.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for Cystatin SN Overexpression Lysate

  • CST1
  • cystain-SA-I
  • cystatin 1
  • cystatin SA-I
  • Cystatin SN
  • cystatin-1
  • cystatin-SN
  • Salivary cystatin-SA-1
  • type 2 family

Background

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a cysteine proteinase inhibitor found in saliva, tears, urine, and seminal fluid. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Cystatin SN Lysate (NBP2-10786) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Cystatin SN Lysate (NBP2-10786). (Showing 1 - 1 of 1 FAQ).

  1. I notice you sell both antibodies and recombinant protein for human cystatin SN. Could you perhaps develop an ELISA for this protein?
    • NBP1-55995 and H00001469-B01P are both suitable for use as antibodies in ELISA. While a kit is not in development, you could use these to make your own ELISA kit, as many of our users do.

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Bioinformatics

Gene Symbol CST1