![Western Blot: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - Analysis of extracts (35ug) from 2 cell lines lysates by using anti-CTAG1B monoclonal antibody (1:500).](http://images.novusbio.com/fullsize/CTAG1B-Antibody-OTI2B6-Azide-and-BSA-Free-Western-Blot-NBP2-70485-img0002.jpg "Western Blot: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - Analysis of extracts (35ug) from 2 cell lines lysates by using anti-CTAG1B monoclonal antibody (1:500).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI2B6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full-length protein expressed in 293T cell transfected with human CTAG1B expression vector |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CTAG1B |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 18 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CTAG1B |
![Immunocytochemistry/Immunofluorescence: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - Staining of 293T cells transfected by pCMV6-ENTRY CTAG1B(RC213318) using anti-CTAG1B antibody (green, upper left; DAPI/blue, lower left; merged, upper right). 293T cells transfected with empty vector served as a negative control (merged, lower right). (1:](http://images.novusbio.com/fullsize/CTAG1B-Antibody-OTI2B6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70485-img0006.jpg "Immunocytochemistry/Immunofluorescence: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - Staining of 293T cells transfected by pCMV6-ENTRY CTAG1B(RC213318) using anti-CTAG1B antibody (green, upper left; DAPI/blue, lower left; merged, upper right). 293T cells transfected with empty vector served as a negative control (merged, lower right). (1:")
![Immunohistochemistry: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-CTAG1Bmouse monoclonal antibody.](http://images.novusbio.com/fullsize/CTAG1B-Antibody-OTI2B6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70485-img0015.jpg "Immunohistochemistry: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-CTAG1Bmouse monoclonal antibody.")
![Flow Cytometry: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-CTAG1B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CTAG1B-Antibody-OTI2B6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70485-img0014.jpg "Flow Cytometry: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-CTAG1B mouse monoclonal antibody.")
![Western Blot: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTAG1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTAG1B.](http://images.novusbio.com/fullsize/CTAG1B-Antibody-OTI2B6-Azide-and-BSA-Free-Western-Blot-NBP2-70485-img0001.jpg "Western Blot: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTAG1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTAG1B.")
![Immunohistochemistry: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-CTAG1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min, NBP1-47714, Dilution 1:50)](http://images.novusbio.com/fullsize/CTAG1B-Antibody-OTI2B6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70485-img0007.jpg "Immunohistochemistry: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-CTAG1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min, NBP1-47714, Dilution 1:50)")
![Immunohistochemistry: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-CTAG1B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CTAG1B-Antibody-OTI2B6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70485-img0008.jpg "Immunohistochemistry: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-CTAG1B mouse monoclonal antibody.")
![Immunohistochemistry: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-CTAG1B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CTAG1B-Antibody-OTI2B6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70485-img0009.jpg "Immunohistochemistry: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-CTAG1B mouse monoclonal antibody.")
![Immunohistochemistry: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-CTAG1B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CTAG1B-Antibody-OTI2B6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70485-img0010.jpg "Immunohistochemistry: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-CTAG1B mouse monoclonal antibody.")
![Immunohistochemistry: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - Staining of paraffin-embedded Carcinoma of kidney tissue using anti-CTAG1B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CTAG1B-Antibody-OTI2B6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70485-img0011.jpg "Immunohistochemistry: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - Staining of paraffin-embedded Carcinoma of kidney tissue using anti-CTAG1B mouse monoclonal antibody.")
![Immunohistochemistry: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - Staining of paraffin-embedded Carcinoma of lung tissue using anti-CTAG1B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CTAG1B-Antibody-OTI2B6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70485-img0012.jpg "Immunohistochemistry: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - Staining of paraffin-embedded Carcinoma of lung tissue using anti-CTAG1B mouse monoclonal antibody.")
![Immunohistochemistry: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - Staining of paraffin-embedded Carcinoma of pancreas tissue using anti-CTAG1B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CTAG1B-Antibody-OTI2B6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70485-img0013.jpg "Immunohistochemistry: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - Staining of paraffin-embedded Carcinoma of pancreas tissue using anti-CTAG1B mouse monoclonal antibody.")
![Flow Cytometry: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - Analysis of living 293T cells transfected with CTAG1B overexpression plasmid (RC213318), Red)/empty vector (Blue) using anti-CTAG1B antibody. Cells incubated with a non-specific antibody (Green) were used as isotype control. (1:100)](http://images.novusbio.com/fullsize/CTAG1B-Antibody-OTI2B6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70485-img0003.jpg "Flow Cytometry: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - Analysis of living 293T cells transfected with CTAG1B overexpression plasmid (RC213318), Red)/empty vector (Blue) using anti-CTAG1B antibody. Cells incubated with a non-specific antibody (Green) were used as isotype control. (1:100)")
![Flow Cytometry: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - Analysis of living NCI-H1299 cells, using anti-CTAG1B antibody(red), compared to an isotype control (green).(1:100)](http://images.novusbio.com/fullsize/CTAG1B-Antibody-OTI2B6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70485-img0004.jpg "Flow Cytometry: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - Analysis of living NCI-H1299 cells, using anti-CTAG1B antibody(red), compared to an isotype control (green).(1:100)")
![Flow Cytometry: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - Analysis of living A375 cells, using anti-CTAG1B antibody(red), compared to an isotype control (green).(1:100)](http://images.novusbio.com/fullsize/CTAG1B-Antibody-OTI2B6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70485-img0005.jpg "Flow Cytometry: CTAG1B Antibody (OTI2B6) - Azide and BSA Free [NBP2-70485] - Analysis of living A375 cells, using anti-CTAG1B antibody(red), compared to an isotype control (green).(1:100)")
![N/A Kallikrein 3/PSA [HRP]](https://images.novusbio.com/images2/DATA_Kallikrein_3_DKK300_ELISA_729.jpg)
![N/A Kallikrein 3/PSA [HRP]](https://images.novusbio.com/images2/DATA_Kallikrein_3_DKK300_ELISA_730.jpg)
![N/A Kallikrein 3/PSA [HRP]](https://images.novusbio.com/images2/Kallikrein_3_DKK300_ELISA_124.jpg)
![Bioactivity IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFNgamma_285IF_2848.jpg)
![SDS-PAGE IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFN-gamma_285-IF_230.jpg)
![SEC-MALS IFN-gamma [Unconjugated]](https://images.novusbio.com/images/285-if_recombinant-human-ifn-gamma-protein-sec-mals-1520239228..jpg)
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")