Independent Antibodies: Western Blot: CROT Antibody [NBP1-85501] - Analysis using Anti-CROT antibody NBP1-85501 (A) shows similar pattern to independent antibody NBP1-85502 (B).
Immunocytochemistry/ Immunofluorescence: CROT Antibody [NBP1-85501] - Immunofluorescent staining of human cell line A-431 shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CROT Antibody [NBP1-85501] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
This antibody was developed against Recombinant Protein corresponding to amino acids: KVPVHKVGNTPLDMNQFRMLFSTCKVPGITRDSIMNYFRTESEGRSPNHIVVLCRGRAFVFDVIHEGCLVTPPELLRQLTYIHKKCHSEPDGPGIAALTSEERTRWAKAREYLIGLD
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CROT
Purity
Immunogen affinity purified
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Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (86%)
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified
Alternate Names for CROT Antibody
carnitine O-octanoyltransferase
COTperoxisomal carnitine O-octanoyltransferase
EC 2.3.1
EC 2.3.1.137
peroxisomal carnitine acyltransferase
peroxisomal carnitine octanoyltransferase
Background
Carnitine octanoyltransferase (EC 2.3.1.137) is a carnitine acyltransferase that catalyzes the reversible transfer of fatty acyl groups between CoA and carnitine. This provides a crucial step in the transport of medium- and long-chain acyl-CoA out of the mammalian peroxisome to the cytosol and mitochondria. See also CRAT (MIM 600184). Van der Leij et al. (2000) [PubMed 11001805] reviewed the function, structural features, and phylogenetics of human carnitine acyltransferase genes, including CROT.[supplied by OMIM]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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