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CRISP-2 Overexpression Lysate

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Western Blot: CRISP2 Overexpression Lysate (Adult Normal) [NBL1-09480] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CRISP2.
Western Blot: CRISP2 Overexpression Lysate (Adult Normal) [NBL1-09480] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CRISP2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

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CRISP-2 Overexpression Lysate Summary

Description

CRISP-2 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC205312

Accession#: NM_003296

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
CRISP2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
27.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for CRISP-2 Overexpression Lysate

  • Cancer/testis antigen 36
  • CRISP2
  • CRISP-2
  • CRISP-2GAPDL5
  • CT36TSP1
  • cysteine-rich secretory protein 2
  • GAPDL5
  • glyceraldehyde-3-phosphate dehydrogenase-like 5
  • testis specific protein 1 (probe H4-1 p3-1)
  • Testis-specific protein TPX-1
  • TPX1
  • TPX1MGC111136
  • TSP1

Background

CRISP2, or Cysteine-rich secretory protein 2, consists of a 243 amino acid isoform that is 27 kDa, and is involved in the regulation of calcium fluxation and ion channel activity during sperm capacitation. Current research is being conducted on CRISP2 and its relation to several diseases and disorders, including male infertility, atopic dermatitis, and sinusitis. The protein is not currently linked to any specific pathways or biological processes, but it interacts with PPP2R1A and PPP2R1B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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CRISP-2
Overexpression Lysate
NBL1-09480
CRISP-2 Overexpression Lysate
Species: Hu
Applications: WB

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Bioinformatics

Gene Symbol CRISP2