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Human Connexin 26/GJB2 ELISA Kit (Colorimetric)

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ELISA: Human Connexin 26/GJB2 ELISA Kit (Colorimetric) [NBP2-79811] - Samples were spiked with high concentrations of Human Connexin 26/GJB2 and diluted with Reference Standard & Sample Diluent to produce samples with ...read more
ELISA: Human Connexin 26/GJB2 ELISA Kit (Colorimetric) [NBP2-79811] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, and other biological fluids.
Standard Curve Range
0.31-20 ng/mL
Sensitivity
0.19 ng/mL

Order Details

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Human Connexin 26/GJB2 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human CX26 in samples. No Significant cross-reactivity or interference between Human CX26 and analogues was observed.
Standard Curve Range
0.31-20 ng/mL
Sensitivity
0.19 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.86%
Intra-Assay
CV% < 5.91%
Spike Recovery
91-108%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
GJB2

Applications/Dilutions

Dilutions
  • ELISA
Publications
Read Publication using NBP2-79811.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Connexin 26/GJB2 ELISA Kit (Colorimetric)

  • beta 2, 26kD (connexin 26)
  • Connexin 26
  • CX26
  • CX26DFNA3
  • DFNA3
  • DFNA3A
  • DFNB1
  • gap junction protein, beta 2, 26kDa (connexin 26)
  • gap junction protein, beta 2, 26kDa
  • GJB2
  • HID
  • KID
  • NSRD1
  • PPK

Background

Connexin-26 / GJB2 is a gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexins, through which materials of low molecular weight diffuse from one cell to a neighboring cell. Each connexin is composed of a hexamer of connexin proteins. Connexins are a multi-gene family of highly related proteins. At least a dozen distinct connexin genes have been identified and many are expressed in a tissue-specific manner. Two distinct lineages, class I (beta) and class II (alpha), have been identified in mammals. Connexin-26 belongs to the class 1 (beta) group of connexins. Mutations in Connexin-26 are associated with genetically derived hearing impairments, including autosomal recessive non syndromic deafness.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for Connexin 26/GJB2 ELISA Kit (NBP2-79811)(1)

We have publications tested in 1 application: ELISA.


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Bioinformatics

Gene Symbol GJB2