![Western Blot: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Western blot analysis of Collagen VI alpha 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-SD83-03-Western-Blot-NBP2-67825-img0009.jpg "Western Blot: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Western blot analysis of Collagen VI alpha 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | SD83-03 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Recombinant protein within Human Collagen VI alpha 1 aa 17-255 / 1,028. (SwissProt: P12109 Human; SwissProt: P12110 Human; SwissProt: P12111 Human; SwissProt: Q02788 Mouse; SwissProt: Q04857 Mouse; Unigene:232118 Rat) |
| Localization | Extracellular matrix. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | COL6A1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Immunocytochemistry Osteopontin/OPN Antibody [Unconjugated]](https://images.novusbio.com/images2/Osteopontin_AF808_Immunocytochemistry__Immunofluorescence_23325.jpg)
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Secondary Antibodies |
Isotype Controls |
Research Areas for Collagen VI alpha 1 Antibody (NBP2-67825)Find related products by research area.
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| Gene Symbol | COL6A1 |
![Immunocytochemistry/Immunofluorescence: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Staining Collagen VI in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-SD83-03-Immunocytochemistry-Immunofluorescence-NBP2-67825-img0008.jpg "Immunocytochemistry/Immunofluorescence: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Staining Collagen VI in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Immunofluorescence analysis of paraffin-embedded mouse colon tissue labeling Collagen VI alpha 1 with Rabbit anti-Collagen VI alpha 1 antibody washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-SD83-03-Immunohistochemistry-Paraffin-NBP2-67825-img0014.jpg "Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Immunofluorescence analysis of paraffin-embedded mouse colon tissue labeling Collagen VI alpha 1 with Rabbit anti-Collagen VI alpha 1 antibody washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Western Blot: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Analysis of Collagen VI on different lysates using anti-Collagen VI antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse heart Lane 2: Human heart Lane 3: Human kidney](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-SD83-03-Western-Blot-NBP2-67825-img0007.jpg "Western Blot: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Analysis of Collagen VI on different lysates using anti-Collagen VI antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse heart Lane 2: Human heart Lane 3: Human kidney")
![Immunocytochemistry/Immunofluorescence: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Staining Collagen VI in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-SD83-03-Immunocytochemistry-Immunofluorescence-NBP2-67825-img0001.jpg "Immunocytochemistry/Immunofluorescence: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Staining Collagen VI in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Staining Collagen VI in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-SD83-03-Immunocytochemistry-Immunofluorescence-NBP2-67825-img0002.jpg "Immunocytochemistry/Immunofluorescence: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Staining Collagen VI in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Staining Collagen VI in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-SD83-03-Immunocytochemistry-Immunofluorescence-NBP2-67825-img0003.jpg "Immunocytochemistry/Immunofluorescence: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Staining Collagen VI in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Analysis of paraffin-embedded human liver tissue using anti-Collagen VI antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-SD83-03-Immunohistochemistry-Paraffin-NBP2-67825-img0004.jpg "Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Analysis of paraffin-embedded human liver tissue using anti-Collagen VI antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Analysis of paraffin-embedded human lung tissue using anti-Collagen VI antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-SD83-03-Immunohistochemistry-Paraffin-NBP2-67825-img0005.jpg "Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Analysis of paraffin-embedded human lung tissue using anti-Collagen VI antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Analysis of paraffin-embedded mouse colon tissue using anti-Collagen VI antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-SD83-03-Immunohistochemistry-Paraffin-NBP2-67825-img0006.jpg "Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Analysis of paraffin-embedded mouse colon tissue using anti-Collagen VI antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Analysis of paraffin-embedded human liver tissue with Rabbit anti-Collagen VI antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-SD83-03-Immunohistochemistry-Paraffin-NBP2-67825-img0010.jpg "Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Analysis of paraffin-embedded human liver tissue with Rabbit anti-Collagen VI antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Analysis of paraffin-embedded human colon tissue with Rabbit anti-Collagen VI alpha 1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-SD83-03-Immunohistochemistry-Paraffin-NBP2-67825-img0011.jpg "Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Analysis of paraffin-embedded human colon tissue with Rabbit anti-Collagen VI alpha 1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Analysis of paraffin-embedded human skin tissue with Rabbit anti-Collagen VI antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-SD83-03-Immunohistochemistry-Paraffin-NBP2-67825-img0012.jpg "Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Analysis of paraffin-embedded human skin tissue with Rabbit anti-Collagen VI antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Collagen VI with Rabbit anti-Collagen VI antibody washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Collagen-VI-alpha-1-Antibody-SD83-03-Immunohistochemistry-Paraffin-NBP2-67825-img0013.jpg "Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Collagen VI with Rabbit anti-Collagen VI antibody washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
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