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CISH/CIS-1 Antibody (989616) [Unconjugated]

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Western blot shows lysates of human lung tissue and human kidney tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human CIS-1 Monoclonal Antibody (Catalog # MAB3194) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CIS-1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human lung tissue and human kidney tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human CIS-1 Monoclonal Antibody (Catalog # MAB3194) followed by HRP-conjugated Anti-Rat IgG ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
989616
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB3194-SP
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CISH/CIS-1 Antibody (989616) [Unconjugated] Summary

Immunogen
E.coli-derived recombinant human CIS-1
Leu11-Leu258
Accession # Q9NSE2
Specificity
Detects human CIS-1 in direct ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Purity Statement
Protein A or G purified from hybridoma culture supernatant
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Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CISH/CIS-1 Antibody (989616) [Unconjugated]

  • CIS-1
  • CIS-1cytokine-inducible SH2-containing protein
  • CIScytokine-inducible inhibitor of signaling type 1B
  • CISH
  • cytokine inducible SH2-containing protein
  • G18Protein G18
  • SOCS
  • Suppressor of cytokine signaling

Background

Cytokine Inducible SH2-containing protein (CIS-1) is a 29 kDa protein found in a variety of cell types. Mono or polyubiquitination generally results in a 37 or 45 kDa molecule. CIS-1 binds to phosphorylated cytokine receptors IL-3 R beta and EPO-R and blocks downstream activation of STAT5 via receptor internalization and ubiquitin‑mediated proteosomal degradation. Human CIS-1 is a 258 aa peptide that contains one SH2 domain (aa 82‑163) and one SOCS box (aa 218‑258). There are two known alternatively spliced variants with a 7- or 13-aa substitution for the 7 N-terminal amino acid residues. Over the region used as immunogen, human CIS-1 is 91% identical to the corresponding mouse and canine protein sequences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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