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CHD3 Antibody

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Immunohistochemistry-Paraffin: CHD3 Antibody [NBP2-14479] - Staining of human cerebral cortex shows distinct nuclear positivity in neuronal cells.
Immunohistochemistry-Paraffin: CHD3 Antibody [NBP2-14479] - Staining of human cerebral cortex shows distinct nuclear positivity in neuronal cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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CHD3 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: LKIKLGLLGGKREKGGSYVFQSDEGPEPEAEESDLDSGSVHSASGRPDGPVRTKKLKRGRPGRKKKKLLGCPA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CHD3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
CHD3 Protein (NBP2-14479PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (89%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CHD3 Antibody

  • ATP-dependent helicase CHD3
  • CHD-3
  • chromodomain helicase DNA binding protein 3
  • chromodomain-helicase-DNA-binding protein 3
  • EC 3.6.1
  • EC 3.6.4.12
  • hZFH
  • Mi-2 autoantigen 240 kDa protein
  • Mi-2a
  • Mi2-ALPHA
  • ZFH
  • Zinc finger helicase
  • zinc-finger helicase (Snf2-like)

Background

CHD3 is a member of the CHD (chromodomain-helicase-DNA-binding) family of proteins that interacts with nucleosomes and plays a role in chromatin remodeling to modulate transcription. The members of the CHD family of proteins possess 3 common structural and functional domains: a chromodomain (chromatin organization modifier), an SNF2-like helicase/ATPase domain, and a C-terminal DNA-binding domain. CHD3 is a key component of the NuRD (nucleosomal remodeling) complex and directly interacts with histone deacetylases 1 and 2, ATR, and TRIM 27. Studies in several species suggest a role for CHD3 in the repression of developmental genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CHD3