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CD99 Antibody [Unconjugated]

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Mouse splenocytes were stained with Goat Anti-Mouse CD99 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3905, filled histogram) or control antibody (Catalog #AB‑108‑C, open histogram), followed by NorthernLights™ 637-conjugated Anti-Goat IgG Secondary Antibody (Catalog # NL002).
Mouse splenocytes were stained with Goat Anti-Mouse CD99 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3905, filled histogram) or control antibody (Catalog #AB‑108‑C, open histogram), followed by ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Phycoerythrin
FAB3905P
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB3905P
Carrier Free
AF3905-SP
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CD99 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD99
Gly25-Leu138
Accession # Q8VCN6
Specificity
Detects mouse CD99 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CD99 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CD99
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF3905 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD99 Antibody [Unconjugated]

  • 12E7
  • CD99 antigenY homolog
  • CD99 molecule
  • CD99
  • E2 antigen
  • HBA71
  • MIC2 (monoclonal 12E7)
  • MIC2
  • MIC2Y
  • MSK5X
  • pilr-1
  • PILR-L
  • Protein MIC2
  • surface antigen MIC2
  • T-cell surface glycoprotein E2

Background

CD99 (also known as PILR-L and MIC2) is a 125 amino acid, 24‑30 kDa glycosylated transmembrane glycoprotein expressed as a homodimer on the surface of endothelial, T and B cells, monocytes and stem cells. CD99 has been implicated in processes like homotypic cell adhesion, diapedesis and oncosupression. The extracellular domain of the mouse CD99 protein used as immunogen is 45% identical to the human counterpart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD99 Antibody (AF3905)(4)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Flow Cytometry, IHC, Western Blot.


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Flow Cytometry
(2)
IHC
(1)
Western Blot
(1)
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Product General Protocols

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Bioinformatics

Gene Symbol CD99
Uniprot