Human CD97 ELISA Kit (Colorimetric) Summary
Standard Curve Range |
23.44-1500 pg/mL (example only; lot dependent) |
Sensitivity |
15.80 pg/mL |
Assay Type |
Sandwich ELISA |
Inter-Assay |
CV% < 10.7 |
Intra-Assay |
CV% < 5.3 |
Spike Recovery |
85-87% |
Sample Volume |
100 ul |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
ADGRE5 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- 96 well microplate coated with capture antibody
- Color Reagent A
- Color Reagent B
- Detection Antibody conjugated to HRP
- Dilution Buffer Concentration
- Sample Dilution Buffer Concentrate
- Standards
- Stop Solution
- Wash Buffer Concentrate
|
Alternate Names for Human CD97 ELISA Kit (Colorimetric)
Background
CD97, a CD55 Receptor, is a leukocyte antigen located on most activated leukocytes. CD97 is highly homologous to EMR2 with five EGF-like domains in the N-terminal extracellular domain, suggesting that it may be involved in cell signaling as well as in cell adhesion (Hamann et al., 1996). Two variants are produced by alternative splicing, where the shorter form lacks exons 5 and 6 and reduces the number of EGF-like domains from 5 to 3, compared to the full-length isoform. CD97 is associated with with the activated lesions of multiple sclerosis. CD97 expression is also implicated in the dedifferentiation of colon and thyroid tumors. CD97 is expressed abundantly in cells of hematopoietic origin and is markedly upregulated on activated T and B cells. ESTs have been isolated from a wide variety of tissue libraries.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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