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CD9 Antibody (10N2L6)

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Western Blot: CD9 Antibody (10N2L6) [NBP3-15593] - Analysis of extracts of various cell lines, using CD9 antibody (NBP3-15593) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Western Blot: CD9 Antibody (10N2L6) [NBP3-15593] - Analysis of extracts of various cell lines, using CD9 antibody (NBP3-15593) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. ...read more
Immunohistochemistry-Paraffin: CD9 Antibody (10N2L6) [NBP3-15593] - Human lung cancer using CD9 Rabbit mAb (NBP3-15593) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 ...read more
Immunoprecipitation: CD9 Antibody (10N2L6) [NBP3-15593] - Analysis of 300ug extracts of THP-1 cells using 3ug CD9 antibody (NBP3-15593). Western blot was performed from the immunoprecipitate using CD9 antibody ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IP
Clone
10N2L6
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CD9 Antibody (10N2L6) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 129-228 of human CD9 (P21926). YNKLKTKDEPQRETLKAIHYALNCCGLAGGVEQFISDICPKKDVLETFTVKSCPDAIKEVFDNKFHIIGAVGIGIAVVMIFGMIFSMILCCAIRRNREMV
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CD9
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:500 - 1:1000
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for CD9 Antibody (10N2L6)

  • BA2
  • BA-2/p24 antigen
  • BTCC-1
  • CD9 antigen
  • CD9 molecule
  • CD9
  • Cell growth-inhibiting gene 2 protein
  • DRAP-27
  • Leukocyte antigen MIC3
  • MIC3
  • motility related protein-1,5H9 antigen
  • Motility-related protein
  • MRP-1FLJ99568
  • p24
  • tetraspanin-29
  • TSPAN29
  • TSPAN-29
  • TSPAN29MIC3CD9 antigen (p24)

Background

CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. It belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). It also regulates cell motility in a variety of cell lines, and appears to be an important regulator of Schwann cell behavior in peripheral nerve.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CD9