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CD72 Antibody [Biotin]

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CD72 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse CD72 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1279) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
CD72 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse CD72 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1279) at 15 µg/mL for 3 hours at room temperature. Cells were ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
AF1279
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF1279
Carrier Free
AF1279
Carrier Free
AF1279-SP
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CD72 Antibody [Biotin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD72
Arg117-Asn354
Accession # P21855
Specificity
Detects mouse CD72 in Western blots. In Western blots, less than 5% cross‑reactivity with recombinant human CD72 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CD72
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Readout System

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD72 Antibody [Biotin]

  • CD72 antigenB-cell differentiation antigen CD72
  • CD72 molecule
  • CD72
  • CD72b
  • Ly-19.2
  • Ly-32.2
  • Lyb-2
  • LYB2lyb-2

Background

CD72, also named B-cell differentiation antigen Lyb-2, is a Type II transmembrane protein with a C-type lectin domain. It associates with CD5 and is a functional receptor for Semaphorin 4D in the immune system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CD72
Uniprot