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CD47 Antibody (472603) [Unconjugated]

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Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD47 Monoclonal Antibody (Catalog # MAB4670, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD47 Monoclonal Antibody (Catalog # MAB4670, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by ...read more
In afunctional flowcytometry test,biotinylated recombinant Human SIRP alpha /CD172a (100ng/mL, Catalog #4546-SA) binds to Human CD47-transfected HEK293 human embryonic kidney cell line (black dotted line). Binding is ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, B/N, CyTOF-ready
Clone
472603
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB4670U-100UG
Alexa Fluor 405
FAB4670V-100UG
Alexa Fluor 488
FAB4670G-100UG
Alexa Fluor 532
FAB4670X
Alexa Fluor 594
FAB4670T-100UG
Alexa Fluor 647
FAB4670R-100UG
Alexa Fluor 700
FAB4670N-100UG
Alexa Fluor 750
FAB4670S-100UG
Allophycocyanin
FAB4670A
Allophycocyanin/Cy7
FAB4670APCCY7
Biotin
FAB4670B
CoraFluor 1
FAB4670CL1
DyLight 350
FAB4670D
DyLight 405
FAB4670E
DyLight 488
FAB4670K
DyLight 550
FAB4670L
DyLight 594
FAB4670M
DyLight 650
FAB4670W
DyLight 680
FAB4670Y
DyLight 755
FAB4670Z
FITC
FAB4670F
HRP
FAB4670H
Janelia Fluor 525
FAB4670JF525
Janelia Fluor 549
FAB4670I
Janelia Fluor 585
FAB4670JF585
Janelia Fluor 635
FAB4670JF635
Janelia Fluor 646
FAB4670J
Janelia Fluor 669
FAB4670JF669
PE/Atto594
FAB4670PEATT594
PE/Cy5.5
FAB4670PECY55
PE/Cy7
FAB4670PECY7
PerCP
FAB4670C
Phycoerythrin
FAB4670P
mFluor Violet 450 SE
FAB4670MFV450
mFluor Violet 500 SE
FAB4670MFV500
mFluor Violet 610 SE
FAB4670MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB4670P
Carrier Free
FAB4670A
Carrier Free
MAB4670-SP
Carrier Free
FAB4670V-100UG
Carrier Free
FAB4670U-100UG
Carrier Free
FAB4670T-100UG
Carrier Free
FAB4670S-100UG
Carrier Free
FAB4670R-100UG
Carrier Free
FAB4670N-100UG
Carrier Free
FAB4670G-100UG
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CD47 Antibody (472603) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD47
Gln19-Pro139
Accession # Q08722
Specificity
Detects human CD47. Stains human CD47 transfectants but not irrelevant transfectants.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD47
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
Publications
Read Publication using
MAB4670 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD47 Antibody (472603) [Unconjugated]

  • antigen identified by monoclonal 1D8
  • Antigenic surface determinant protein OA3
  • CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
  • CD47 antigen
  • CD47 glycoprotein
  • CD47 molecule
  • CD47
  • IAP
  • IAPintegrin associated protein
  • Integrin-associated protein
  • leukocyte surface antigen CD47
  • MER6
  • MER6integrin-associated signal transducer
  • OA3
  • Protein MER6
  • Rh-related antigen

Background

CD47, also known as integrin-associated protein, is a ubiquitous 50 kDa multipass transmembrane protein with a single IgV-like domain at its N-terminus. CD47 binding to SIRP alpha prevents the phagocytic engulfment of viable cells. Thrombospondin interaction with CD47 on T cells reduces T cell proliferation and inflammatory reactions. Alternate splicing generates isoforms with truncated cytoplasmic domains. Within the N-terminal ECD, human CD47 shares 63% aa sequence identity with mouse and rat CD47.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CD47 Antibody
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CD47 Antibody (472603) [Unconjugated]
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Publications for CD47 Antibody (MAB4670)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC, Inhibition.


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IHC
(1)
Inhibition
(1)
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Bioinformatics

Gene Symbol CD47
Uniprot