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CD40/TNFRSF5 Antibody (1C10) [Alexa Fluor® 405]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
1C10
Clonality
Monoclonal
Host
Rat
Conjugate
Alexa Fluor 405
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB440U-100UG
Alexa Fluor 488
FAB440G-100UG
Alexa Fluor 532
FAB440X
Alexa Fluor 594
FAB440T-100UG
Alexa Fluor 647
FAB440R-100UG
Alexa Fluor 700
FAB440N-100UG
Alexa Fluor 750
FAB440S-100UG
Allophycocyanin/Cy7
FAB440APCCY7
Biotin
BAM440
CoraFluor 1
FAB440CL1
DyLight 350
FAB440D
DyLight 405
FAB440E
DyLight 488
FAB440K
DyLight 550
FAB440L
DyLight 594
FAB440M
DyLight 650
FAB440W
DyLight 680
FAB440Y
DyLight 755
FAB440Z
Fluorescein
FAB440F
HRP
FAB440H
Janelia Fluor 525
FAB440JF525
Janelia Fluor 549
FAB440I
Janelia Fluor 585
FAB440JF585
Janelia Fluor 635
FAB440JF635
Janelia Fluor 646
FAB440J
Janelia Fluor 669
FAB440JF669
PE/Atto594
FAB440PEATT594
PE/Cy5.5
FAB440PECY55
PE/Cy7
FAB440PECY7
Unconjugated
MAB440-100
mFluor Violet 450 SE
FAB440MFV450
mFluor Violet 500 SE
FAB440MFV500
mFluor Violet 610 SE
FAB440MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB440-100
Carrier Free
FAB440F
Carrier Free
MAB440-SP
Carrier Free
MAB440-500
Carrier Free
MAB440-100
Carrier Free
FAB440U-100UG
Carrier Free
FAB440T-100UG
Carrier Free
FAB440S-100UG
Carrier Free
FAB440R-100UG
Carrier Free
FAB440N-100UG
Carrier Free
FAB440G-100UG
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CD40/TNFRSF5 Antibody (1C10) [Alexa Fluor® 405] Summary

Immunogen
COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant mouse CD40/TNFRSF5
Extracellular domain
Specificity
Specifically binds mouse CD40/TNFRSF5 expressed on L cells.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
CD40
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Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD40/TNFRSF5 Antibody (1C10) [Alexa Fluor® 405]

  • B cell surface antigen CD40
  • B-cell surface antigen CD40
  • Bp50B cell-associated molecule
  • CD40 antigen
  • CD40 molecule, TNF receptor superfamily member 5
  • CD40 type II isoform
  • CD40
  • CD40L receptor
  • CDw40
  • MGC9013
  • nerve growth factor receptor-related B-lymphocyte activation molecule
  • p50
  • TNFRSF5
  • TNFRSF5CD40 antigen (TNF receptor superfamily member 5)
  • tumor necrosis factor receptor superfamily member 5
  • tumor necrosis factor receptor superfamily, member 5

Background

CD40 is a type I transmembrane glycoprotein belonging to the TNF receptor superfamily. The mature mCD40 consists of a 172 amino acid (aa) extracellular domain, a 22 aa transmembrane region and a 90 aa cytoplasmic domain (1). CD40 is expressed on B cells, follicular dendritic cells, dendritic cells, activated monocytes, macrophages, endothelial cells, vascular smooth muscle cells and several tumor cell lines (2). The extracellular domain has the cysteine-rich repeat regions, which are characteristic for many of the receptors of the TNF superfamily. Interaction of CD40 with its ligand, CD40L, leads to the aggregation of CD40 molecules, which in turn interact with cytoplasmic components to initiate signaling pathways. Early studies on the CD40-CD40L system revealed its role in humoral immunity. Interaction between CD40L on T cells and CD40 on B cells stimulated B cell proliferation and provided the signal for immunoglobulin isotype switching (3). Mutations in the CD40L gene, which resulted in a CD40L molecule unable to interact with CD40, are responsible for the hyper-IgM syndrome (4). Cross-linking of CD40 with antibodies or by binding to CD40L produces cell type-specific responses which include costimulation and induction of proliferation, induction of cytokine production, rescue from apoptosis, and upregulation of adhesion molecules (5). Some of the early events of intracellular signaling by the CD40-CD40L system include the association of the CD40 with TRAFs and the activation of various kinases (6‑8).

  1. Torres, R.M. and E.A. Clark (1992) J. Immunol. 148:620.
  2. Schonbeck, U. et al. (1997) J. Biol. Chem. 272:19569.
  3. Armitage, R.J. et al. (1993) J. Immunol. 150:3671.
  4. Callard, R.E. et al. (1993) Immunol. Today 14:559.
  5. Stout, R.D. and J. Suttles (1996) Immunol. Today 17:487.
  6. Pullen, S.S. et al. (1999) Biochemistry 38:10168.
  7. Faris, M. et al. (1994) J. Exp. Med. 179:1923.
  8. Hanissian, S.H. and R.S. Geha (1997) Immunity 6:379.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for CD40/TNFRSF5 Antibody (FAB440V). (Showing 1 - 2 of 2 FAQ).

  1. I wanna know does CD40 antibody for IHC-P comes with positive control slide?
    • Our CD40 antibodies do not come with positive control slides however you can use any type of primary carcinoma cell as a positive control. I recommend breast carcinoma as this is what we have tested our CD40 antibodies on.
  2. We are studying CD40L in our system. It has been known that both CD40 and CD11b are receptors for CD40L. We need CD40 antibody to neutralize CD40 binding to CD40L, then we may prove what is function for CD11b binding to CD40L. Do you have any CD40 Ab which has block function only between CD40 and CD40L, but not between CD11b and CD40L?
    • Unfortunately, none of our CD40 antibodies have been specifically tested for this application.

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Bioinformatics

Gene Symbol CD40