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Mouse CD30 Ligand/TNFSF8 ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA, NULL
Suitable Sample Type
Body Fluid, Cell Culture Supernatant, Serum, Tissue Lysate
Standard Curve Range
15.6 to 1000 pg/mL
Sensitivity
1 pg/mL

Order Details

Mouse CD30 Ligand/TNFSF8 ELISA Kit (Colorimetric) Summary

Description
Quality control test: Standard curve. Tnfsf8 (Mouse) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of mouse Tnfsf8.
Standard Curve Range
15.6 to 1000 pg/mL
Sensitivity
1 pg/mL
Assay Type
Sandwich ELISA
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
TNFSF8

Applications/Dilutions

Dilutions
  • ELISA
  • Quantification
Publications
Read Publications using KA0550.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Kit Components

Components
  1. ABC diluent buffer: 12ml.
  2. Antibody diluent buffer: 12ml.
  3. Avidin-Biotin-Peroxidase Complex (ABC) : 130µl, dilution 1:100.
  4. Biotinylated anti- mouse CD30L antibody : 130µl, dilution 1:100.
  5. Lyophilized recombinant mouse CD30L standard: 10ng/tube×2.
  6. One 96-well plate precoated with anti- mouse CD30L antibody.
  7. Sample diluent buffer: 30 ml
  8. TMB color developing agent: 10ml.
  9. TMB stop solution: 10ml.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Mouse CD30 Ligand/TNFSF8 ELISA Kit (Colorimetric)

  • CD153 antigen
  • CD153
  • CD30 antigen ligand
  • CD30 Ligand
  • CD30L
  • CD30-L
  • CD30LCD30 ligand
  • CD30LGMGC138144
  • TNFSF8
  • tumor necrosis factor (ligand) superfamily, member 8
  • tumor necrosis factor ligand superfamily member 8

Background

CD153 is a 39-40 kD type II membrane glycoprotein, also known as CD30 ligand or CD30L. This TNF superfamily member is expressed on activated T cells. CD153 has been reported to stimulate B-cell proliferation, antigen-specific antibody production, polyclonal immunoglobulin secretion, and T cell activation and proliferation. The RM153 antibody inhibits binding of CD153 to CD30.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for CD30 Ligand/TNFSF8 ELISA Kit (KA0550)(6)

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Bioinformatics

Gene Symbol TNFSF8