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CD177 Antibody (4C4) - Azide and BSA Free

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Western Blot: CD177 Antibody (4C4) [H00057126-M01] - Analysis of CD177 expression in transfected 293T cell line by CD177 monoclonal antibody (M01), clone 4C4.Lane 1: CD177 transfected lysate(46.4 KDa).Lane 2: Non-transfected lysate.
Western Blot: CD177 Antibody (4C4) [H00057126-M01] - Analysis of CD177 expression in transfected 293T cell line by CD177 monoclonal antibody (M01), clone 4C4.Lane 1: CD177 transfected lysate(46.4 KDa).Lane 2: ...read more
Immunohistochemistry-Paraffin: CD177 Antibody (4C4) [H00057126-M01] - Analysis of monoclonal antibody to CD177 on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 3 ug/ml.
Immunoprecipitation: CD177 Antibody (4C4) [H00057126-M01] - Analysis of CD177 transfected lysate using anti-CD177 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CD177 MaxPab rabbit polyclonal ...read more
ELISA: CD177 Antibody (4C4) [H00057126-M01] - Detection limit for recombinant GST tagged CD177 is approximately 3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IP
Clone
4C4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free

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CD177 Antibody (4C4) - Azide and BSA Free Summary

Immunogen
CD177 (AAH29167, 21 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ALLCQFGTVQHVWKVSDLPRQWTPKNTSCDSGLGCQDTLMLIESGPQVSLVLSKGCTEAKDQEPRVTEHRMGPGLSLISYTFVCRQEDFCNNLVNSLPLW
Specificity
CD177 (4C4)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD177
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Publications
Read Publications using H00057126-M01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CD177 Antibody (4C4) - Azide and BSA Free

  • CD177 antigenNB1 GP
  • CD177 molecule
  • CD177
  • HNA-2a
  • HNA2Acell surface receptor
  • Human neutrophil alloantigen 2a
  • NB1 GP
  • NB1PRV-1
  • polycythemia rubra vera 1
  • Polycythemia rubra vera protein 1
  • PRV1
  • PRV1NB1 glycoprotein

Background

NB1, a glycosyl-phosphatidylinositol (GPI)-linked N-glycosylated cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia (Lalezari et al., 1971 [PubMed 5552408]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD177 Antibody (H00057126-M01)(2)


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Bioinformatics

Gene Symbol CD177
Entrez
OMIM
Uniprot