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CCL22/MDC Antibody (158132) [Unconjugated]

 (6 Publications)
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Recombinant Mouse CCL22/MDC (Catalog # 439-MD) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL22/MDC (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse CCL22/MDC Monoclonal Antibody (Catalog # MAB4391). The ND50 is typically 0.4-2.0 µg/mL.
Recombinant Mouse CCL22/MDC (Catalog # 439-MD) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications B/N, ELISA(Cap)
Clone
158132
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB4391-SP
Carrier Free
MAB4391-500
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CCL22/MDC Antibody (158132) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse CCL22/MDC
Gly25-Ser92
Accession # O88430
Specificity
Detects mouse CCL22/MDC in ELISAs and Western blots. In Western blots, this antibody shows 25% cross-reactivity with recombinant viral MIP‑II and no cross-reactivity with rmCCL1, 2, 3, 4, 6, 7, CCL9/10/MIP-1 gamma , 11, 12, 19, 20, 21, 22, 24, 25, 27, rhCCL1, 2, 3, 4, 5, 7, 8, 11, 13, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 28, or rrCCL20.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
CCL22
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.4-2.0 ug/mL
Application Notes
ELISA Detection: Mouse CCL22/MDC Biotinylated Antibody (Catalog number BAF439)
Standard: Recombinant Mouse CCL22/MDC (Catalog number 439-MD)
Publications
Read Publications using
MAB4391 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL22/MDC Antibody (158132) [Unconjugated]

  • A-152E5.1
  • ABCD-1
  • CC chemokine STCP-1
  • C-C motif chemokine 22
  • CCL22
  • chemokine (C-C motif) ligand 22
  • DC/B-CK
  • Macrophage-derived chemokine
  • MDC
  • MDCStimulated T-cell chemotactic protein 1
  • MGC34554
  • SCYA22MDC(1-69)
  • small inducible cytokine A22
  • small inducible cytokine subfamily A (Cys-Cys), member 22
  • Small-inducible cytokine A22
  • STCP-1
  • stimulated T cell chemotactic protein 1

Background

CCL22, also known as ABCD-1 and MDC (macrophage-derived chemokine), is a CC chemokine cloned from activated mouse B cells. Mouse CCL22 cDNA encodes a precursor protein of 92 amino acid (aa) residues with a 24 aa residue predicted signal peptide that is cleaved to yield a 68 aa residue mature 7.8 kDa protein. At the amino acid sequence level, mouse and human CCL22 share 64% identity and 83% similarity. The genomic organization of the mouse and human CCL22 genes are very similar, exhibiting sequence identity at the intron-exon boundaries. Mouse CCL22 is expressed at high levels in dendritic cells and activated B lymphocytes. Low levels of mouse CCL22 mRNA are also detectable in lung, unstimulated spleen cells, lymph node cells and in thymocytes. CCL22 is a functional ligand for the CC chemokine receptor 4. Recombinant or chemically synthesized mature mouse CCL22 has been shown to induce chemotaxis or Ca2+ mobilization in activated mouse and human T cells.

  1. Schaniel, C. et al. (1998) J. Exp. Med. 188:451.
  2. Imai, T. et al. (1998) J. Biol. Chem. 273:1764.
  3. Godiska, R. et al. (1997) J. Exp. Med. 185:1595.
  4. Chang, M-S. et al. (1997) J. Biol. Chem. 272:25229.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CCL22/MDC Antibody
(158132) [Unconjugate ...
MAB4391
CCL22/MDC Antibody (158132) [Unconjugated]
Species: Mu
Applications: B/N, ELISA(Cap)

Publications for CCL22/MDC Antibody (MAB4391)(6)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: ELISA Development, Luminex Development, Neutralization.


Filter By Application
ELISA Development
(2)
Luminex Development
(1)
Neutralization
(1)
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Mouse
(4)
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Showing Publications 1 - 6 of 6.
Publications using MAB4391 Applications Species
Jared Klarquist, Kristen Tobin, Peyman Farhangi Farhangi Oskuei, Steven W. Henning, Manuel F. Fernandez, Emilia R. Dellacecca et al. Ccl22 Diverts T Regulatory Cells and Controls the Growth of Melanoma Cancer Research 2016-11-01 [PMID: 27634754]
Sandra Santulli-Marotto, John Wheeler, Eilyn R. Lacy, Ken Boakye, Jennifer Luongo, Sheng-Jiun Wu et al. CCL22-specific Antibodies Reveal That Engagement of Two Distinct Binding Domains on CCL22 Is Required for CCR4-mediated Function Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 2015-12-20 [PMID: 26683175]
Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012-01-25;58(2):169-77. 2012-01-25 [PMID: 22277799] (Luminex Development, Mouse) Luminex Development Mouse
Kearley J, Buckland KF, Mathie SA, Lloyd CM Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway. Am. J. Respir. Crit. Care Med., 2009-01-29;179(9):772-81. 2009-01-29 [PMID: 19179489] (ELISA Development, Mouse) ELISA Development Mouse
Kearley J, Robinson DS, Lloyd CM CD4+CD25+ regulatory T cells reverse established allergic airway inflammation and prevent airway remodeling. J. Allergy Clin. Immunol., 2008-07-30;122(3):617-24.e6. 2008-07-30 [PMID: 18672278] (ELISA Development, Mouse) ELISA Development Mouse
Meyer EH, Wurbel MA, Staton TL, Pichavant M, Kan MJ, Savage PB, DeKruyff RH, Butcher EC, Campbell JJ, Umetsu DT iNKT cells require CCR4 to localize to the airways and to induce airway hyperreactivity. J. Immunol., 2007-10-01;179(7):4661-71. 2007-10-01 [PMID: 17878364] (Neutralization, Mouse) Neutralization Mouse

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Bioinformatics

Gene Symbol CCL22
Uniprot