![Immunohistochemistry-Paraffin: Boll Antibody [NB300-904] - (4ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining](http://images.novusbio.com/fullsize/Boll-Antibody-Immunohistochemistry-Paraffin-NB300-904-img0002.jpg "Immunohistochemistry-Paraffin: Boll Antibody [NB300-904] - (4ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining")
| Reactivity | Hu, Bv, CaSpecies Glossary |
| Applications | ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-QPEPIKTVWSIHY corresponding to C-Terminus according to NP_932074.1, NP_149019.1. |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_932074.1; NP_149019.1). |
| Predicted Species | Canine (100%), Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | BOLL |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Preliminary experiments gave an approx. 22 kDa band in human testis lysates after 1.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.6 kDa band according to NP_932074.1 and 31.3 kDa band according to NP_149019.1. The 22 kDa band was successfully blocked by incubation with the immunizing peptide. However, literature reports a 36 kDa band in human and mouse testisscientific literature (PMID: 11390979). IHC-P: Human testis shows cytoplasm staining in spermatocytes, but not in spermatogonia. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | BOLL |
| Entrez |
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| Uniprot |
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