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Boll Antibody (5H8)

Images

 
Immunocytochemistry/ Immunofluorescence: Boll Antibody (5H8) [H00066037-M08] - Analysis of monoclonal antibody to BOLL on HeLa cell. Antibody concentration 10 ug/ml
ELISA: Boll Antibody (5H8) [H00066037-M08] - Detection limit for recombinant GST tagged BOLL is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
5H8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Boll Antibody (5H8) Summary

Immunogen
BOLL (NP_149019, 185 a.a. ~ 283 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ATTQYLPGQWQWSVPQPSASSAPFLYLQPSEVIYQPVEIAQDGGCVPPPLSLMETSVPEPYSDHGVQATYHQVYAPSAITMPAPVMQPEPIKTVWSIHY
Specificity
BOLL (5H8)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BOLL
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
Application Notes
Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Boll Antibody (5H8)

  • bol, boule-like (Drosophila)
  • BOULEbol (Drosophila boule homolog)-like
  • protein boule-like

Background

This gene belongs to the DAZ gene family required for germ cell development. It encodes an RNA-binding protein which is more similar to Drosophila Boule than to human proteins encoded by genes DAZ (deleted in azoospermia) or DAZL (deleted in azoospermia-like). Loss of this gene function results in the absence of sperm in semen (azoospermia). Histological studies demonstrated that the primary defect is at the meiotic G2/M transition. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol BOLL
Entrez
OMIM
Uniprot